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Karyotype Analysis in Lignosus Bean (dipogon Lignosus) and Lablab Bean (lablab Purpureus)

M. A. Ali
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. M. Hasan
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Mia
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Q. N. Ahmad
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted in the cytogenetics laboratory of the Department of Genetics and Plant Breeding of Bangladesh Agricultural University during July 2008 to September 2009 aiming to analyze and compare the karyotypes of two genotypes of Lignosus bean (Dipogon lignosus L.) and Lablab bean (Lablab purpureus L.). Root tips from germinated seeds were collected, pretreated and fixed in acetic alcohol for using in slide preparation. Chromosome measurements were done from the prints of the photomicrographs of prometaphase plates. In both the genotypes, 2n = 22 chromosomes was found. The range of length of the individual chromosome was 1.22 to 2.17μ in Lignosus bean, and 1.17 to 3.00μ in Lablab bean. In the Lignosus bean genotype, the haploid complement consisted of 11 metacentric (m) chromosomes with 3 individually identifiable ones but incase of Lablab bean the haploid complement consisted of 11 m chromosomes with 5 individually identifiable ones. All identifiable chromosomes were metacentric. This karyotype analysis of Lignosus bean and Lablab bean will be useful for further genetic studies.

Keywords: Chromosomal characteristics, Karyotype analysis, Bean, Derivation

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Bean

Objectives:

To analyze and compare the karyotypes of the two species in detail with special emphasis on the identification of individual chromosome.

Methodology:

Root tip collection and fixation: Seeds of Lignosus bean (Trinidad) and Country bean (DS-106) were germinated in the laboratory. Healthy and vigorously growing roots of 1.5-2.0cm long were collected and thoroughly washed. The roots were then pretreated in saturated aqueous solution of monobromonaphthalene (MBN) for 70 minutes with occasional stirring. MBN pretreated roots were then thoroughly washed and fixed in freshly prepared acetic alcohol. The fixed roots were left in the fixative until these were used for slide preparation. Slides were prepared within a few days of fixation of the roots. The fixation of roots and slide preparation was repeated as and when needed.

Photomicrography and measurement of chromosomes: Photomicrography of the chromosome plates was done from July 2009 to August 2009 in Post-graduate Laboratory of the Department of Genetics and Plant Breeding. Photomicrographs of the selected chromosome plates were taken with the aid of Olympus research microscope model BX40 using plan 100X objective. Fujicolour 200 ASA DX Film was used. Film processing and printings were made from commercial centres. Chromosome measurements were made from printed images and chromosome length were converted to micron (μ) based on calculated print magnification.

Karyotype analysis: The various steps followed in analysing the karyotypes were closely related to and dependent upon the results. For this reason the different procedural steps and the related discussion involving the method of analysis has been incorporated into the results section.

Source of Information: J. Bangladesh Agril. Univ. 9(1): 27–36, 2011 ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The range of length of the individual chromosome was 1.22 to 2.17μ in Lignosus bean, and 1.17 to 3.00μ in Lablab bean. In the Lignosus bean genotype, the haploid complement consisted of 11 metacentric (m) chromosomes with 3 individually identifiable ones but incase of Lablab bean the haploid complement consisted of 11 m chromosomes with 5 individually identifiable ones. All identifiable chromosomes were metacentric. This karyotype analysis of Lignosus bean and Lablab bean will be useful for further genetic studies.

Knowledge Management: Journal