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Molecular Epidemiologic Study on Rotavirus Infection in Human and Birds in Association with Gastroenteritis

M. S. Islam
Department of Medicine, Bangladesh Agricultural University, Mymensingh

M. M. Alam
Department of Medicine, Bangladesh Agricultural University, Mymensingh

M. U. Ahmed
Department of Medicine, Bangladesh Agricultural University, Mymensingh

A. B. M. Saifuzzaman
Parashuram Veterinary Hospital, Feni

N. Kobayashi
Department of Hygiene, Sapporo Medical University School of Medicine, Japan

M. E. H. Kayesh
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

M. S. Uddin
Department of Medicine, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

A total of 315 stool samples were screened from hospitalized diarrheic human patient and 251 from diarrheic birds from July 2006 to May 2007 by polyacrylamide gel and silver staining (PAGE-SS) technique. Human rotavirus (HRV) and avian rotavirus were detected in 35.28% stool specimens and 13.15% bird’s faecal specimens respectively. The occurrence of rotavirus infection in human patients of 181-365 days age group was relatively high (45.75%) while it was 41.82% in under 5 years old. The birds of 7-12 days age group was found most frequently associated with rotavirus infection and the rate was found 45%.

Keywords: Avian rotavirus, Human rotavirus, PAGE, Eletropherotypes

Location: Mymensingh

Start Date: 01-07-2006

End Date: 31-05-2007

Program Area: Pest Management

Commodity:

Objectives:

Considering the importance of rotavirus associated diarrhoea, its diverse epidemiological features and for rapid detection of rotavirus infection in human and birds has been studied in this research work.

Methodology:

The present study was carried out during the period from July 2006 to May 2007 in the department of Medicine, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. Collection of samples A total of 251 faecal specimens were collected from “Tania” poultry farm, “Jalil” poultry farm, “Babul” poultry farm, the neighboring village poultry farm of BAU Campus, Mymensigh; “Petrol pump” poultry farm, Babuganj, Barisal; “Shaikat” poultry farm, Pazulia, Gazipur and “Pahartauli” poultry farm, Chittagong during the period of July 2006 to May 2007. The faecal specimens were obtained from the cloaca of the broiler birds of 1 to 24 days of age. For each broiler bird, 5-10 gms of sample was collected. Precautions were taken to avoid contamination of one specimen with other. The date of collection, age, clinical signs and environmental history were recorded for each case. After collection the samples were transported to the laboratory and stored at-20⁰C until used for electrophoresis. For convenience of the study, the birds were arranged in four groups on the basis of their age: birds having 1 day to 6 days of age, 7 days to 12 days of age, 13 days to 18 days of age and 19 days to 24 days of age. 315 stool specimens were collected from two hospitals namely S. K. Hospital and Mymensingh Medical College Hospital (MMCH), Mymensingh during the period from July 2006 to May 2007. The freshly voided stool specimens were collected during defecation in sterile screw capped container. After thawing of the stored samples 1 gm of each specimen was taken in a test tube and 9 ml of PBS (pH 7.4) was added. Faeces and PBS was thoroughly suspended by using a spinmix. The suspension was either used freshly for next phase or stored at -20⁰C for future use.

Source of Information: Bangl. J. Vet. Med. (2009). 7(1) : 233 – 237

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

To understand the epidemiology of rotavirus infection in man and birds, however, further epidemiological studies in relation to the association of other causal agents with diarrhoea, the strain of virus, antigenic structures with reference serotypes and zoonotic potential of rotavirus is necessary before introduction of vaccines against rotavirus diarrhoea in Bangladesh. In these regard further studies of both human and avian rotaviruses should be continued in different parts of the country to know the real status of rotavirus infection in Bangladesh, to understand the genetic diversity and evolutionary mechanisms of human rotavirus to develop a strategy for prevention of rotavirus associated diarrhea.

Knowledge Management: Journal