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Rotavirus Infection in Human and Birds

M. M. Alam
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University

M. M. Rahman
Laboratory Medicine, Islami Bank Hospital, Motijhil, Dhaka

M. S. Islam
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University

M. U. Ahmed
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University

Abstract/ Executive Summary:

An epidemiological study on rotavirus infection using 315 stool specimens from hospitalized diarrhoeic human patients and 251 fecal specimens from diarrheic birds (broiler) wear examined by RNA polyacrylamide-gel electrophoresis and silver staining (PAGE-SS) technique. Rotavirus was detected in 35.28% (111/315) stool specimens. The highest rate of rotavirus infection in human was detected in the winter (45.94%), particularly in the month of December to January (44%-51%). The rotavirus diarrhea was found slightly higher in male (35.97%) than female (34.12%). Rotavirus was detected in 13.15% (33/251) birds’ fecal specimens. The birds were affected by the rotavirus, showing only the long RNA electropherotype. The antigenic studies with reference to subgroups and serotypes specificity and its zoonotic potential needs to be studied further.

Keywords: Rotavirus, Diarrhoea , RNA electropherotype and Antigenic structures

Location:

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity:

Objectives:

Considering the importance of rotavirus associated diarrhoea, its diverse epidemiological features and for rapid detection of rotavirus infection in human and birds have been studied in this present research.

Methodology:

A total of 251 faecal specimens were collected from “Tania” poultry farm, “Jalil” poultry farm, “Babul” poultry farm, the neighboring village poultry farm of BAU Campus, Mymensingh; “Petrol pump” poultry farm, Babuganj, Barisal; “Shaikat” poultry farm, Pazulia, Gazipur and “Pahartauli” poultry farm, Chittagong and 315 stool specimens (189 male and 126 female) were collected from two hospitals namely S. K. Hospital and Mymensingh Medical College Hospital (MMCH), Mymensingh during the period from July 2006 to May 2007. The faecal specimens were obtained from the cloaca in case of broiler birds and freshly voided stool specimens were collected during defecation in sterile screw capped container. The date of collection, age, sex, clinical signs, important clinical history and environmental situations were recorded in each case using pre-tested questioners. The faecal specimens were examined for rotavirus by electrophoresis. Viral RNA from stool specimens was extracted as described by Seele and Alexander (1978) and Dimitrov et al. (1984). Electropherotyping of viral RNA was carried out in 10% polyacrylamide slab gels, and silver staining technique as described by Kobayashi et al. (1989). Statistical analysis Statistical analyses were performed using the chi-square test and Fisher’s exact test. The level of significance was set at p<0.05.

Source of Information: Bangl. J. Vet. Med. (2009). 7(2) : 314 – 319

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present study showed some epidemiological parameters of rotavirus infection in man and birds (broiler). However, further epidemiological studies in relation to the strains of virus, antigenic structures with reference to serotypes and zoonotic potential of rotavirus is necessary before introduction of vaccines against rotavirus diarrhea in Bangladesh

Knowledge Management: Journal