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Comparative Immunogenicity of Biotyphoid® and Dls Prepared Fowl Typhoid Vaccine in Layer Chickens

J. Ferdous
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

M. S. R. Khan
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

F. Begum
Veterinary Training Institute, Khakdahor, Mymensingh

J. Hassan
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

B. Sarker
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The experiment was conducted to investigate the immunogenicity of DLS prepared Fowl typhoid vaccine in comparison to commercially available BIO-TYPHOID® (Imported by Advance Animal Science Co. Ltd., Bangladesh) in layer chickens. To compare the immunogenicity of these two vaccines a total of 30 chickens were divided into three groups (group A, B and C) each including 10 layer chickens of Fayoumi breed. Chickens of group A and B were vaccinated with DLS prepared fowl typhoid vaccine and BIO-TYPHOID® respectively following usual schedule of vaccination (i.e. first dose at 42 days of age and second dose at 72 and 112 days for DLS prepared fowl typhoid vaccine and BIO-TYPHOID® respectively) with a dose of 0.5 ml at each occasion through SC route. Birds of group C were kept as unvaccinated control. Blood samples were collected to obtain sera from each chicken at every 7 days interval up to 105 days post vaccination for determination of antibody titre following primary and secondary vaccination using Microplate Agglutination test. Highest mean antibody titres obtained from Group A and B which were 460.80 and 409.60 respectively. Among the chickens vaccinated the highest mean antibody titre of 460.80 was obtained at 56 days post vaccination from chickens in group A vaccinated with DLS prepared Fowl typhoid vaccine. Results of this study revealed that DLS prepared fowl typhoid vaccine induced higher antibody production than BIOTYPHOID .

Keywords: Comparative, immunogenicity, DLS, fowl typhoid, Vaccine, BIO-TYPHOID

Location: Mymensingh

Start Date: 01-01-2008

End Date: 30-06-2008

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To study the immunogenicity of DLS prepared FT vaccine in comparison to a commercially available FT vaccine.

Methodology:

The study was carried out in the Bacteriology laboratory of the Department of Microbiology and Hygiene,Bangladesh Agricultural University (BAU), Mymensingh during the period from January to June, 2008. A total of 30 day–old chicks of Fayoumi breed were collected from the poultry farm of Bangladesh Agricultural University (BAU), Mymensingh. These chicks were reared in the poultry shed of the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh providing commercial Two inactivated fowl typhoid (FT) vaccines were used in this experiment. These are- a) Fowl typhoid vaccine produced by the Department of Livestock Services (DLS) at the Livestock Research Institute (LRI), Mohakhali, Dhaka was obtained from the District Livestock Office, Mymensingh and b) Bio-Typhoid produced by Merial, France, and marketed by Advance Animal Science Co. Ltd. was purchased from the local market. These vaccines were stored at 4-8⁰C until use.feed and water with proper biosecurity.A total of 30 day old chicks were used in this experiment and the birds were divided into 3 groups viz. A, B and C each group containing 10 chicks. The birds of group A and B were vaccinated with DLS prepared FT vaccine and BIO-TYPHOID® following usual schedule of vaccination. Incase of group A, 1st dose of DLS prepared FT vaccine was administered at 42 days of age of the birds, then 2nd dose was administered at 72 days of age. In case of group B, 1st dose of “BIO-TYPHOID was administered at 42 days of age of the birds, then 2nd dose was administered at 112 days of age. The immunization was conducted following the procedure of Heddleston and Reisinger (1960). The birds of group C were kept unvaccinated. The vaccines were administered through SC route @ 0.5 ml per bird.The Microplate Agglutination test was carried out with the sera to determine the presence of specific antibody against S. gallinarum and titre of antibody in the sera. The test was conducted following the procedure of Schlink and Olson (1979). The value reciprocal to the highest dilution at which agglutination occurred was considered as the titre of the serum. The antibody titre was expressed as Geometric mean with standard error.

Source of Information: Bangl. J. Vet. Med. (2010). 8(1): 1–4

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

So, from the above study, it was observed that chickens of group A vaccinated with DLS prepared Fowl typhoid vaccine exhibited higher antibody titre than the chickens in group B vaccinated with BIO-TYPHOID®. The stimulation of higher level of immunity by DLS-FT vaccine might be due to higher immunogenic character of the vaccine strain and use of local isolate of S. gallinarum in DLS-FT vaccine.

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