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A Cross-sectional Study for Mycoplasma Gallisepticum Antibodies in Non-vaccinated Commercial Layer Birds in Khulna District

M. A. Jalil
District Livestock Office, Khulna

M. T. Islam
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

A cross-sectional study was carried out on 96 commercial layer farms of six upazilas of Khulna district to know the seroprevalence of Mycoplasma gallisepticum (MG) infection during the period from August 2009 to July 2010. A total of 1268 sera samples were collected from randomly selected layer birds of different age groups and tested by serum plate agglutination (SPA) test by using commercial MG antigen (Nobilis® MG, Intervet International) to detect the presence of antibodies against MG. The overall prevalence of MG infection was recorded as 67.4%. Age-wise analysis revealed that there was significant relationship (p<0.001) between MG infection and age of birds. The highest prevalence of MG infection was 71.2% in 21-56 weeks age group followed by 66.8% in 8-20 weeks age group. There was significant (p<0.001) variation in the seroprevalence in terms of areas of study (upazilas). Significant (p<0.001) effect of seasons in the seroprevalence of MG infection in layer birds was observed in the present study. The prevalence of MG infection was the highest (75.6%) in winter season followed by summer (66.3%) and rainy (60.8%) seasons. The seroprevalence of MG infection was higher in most of the large flocks and lower in small flocks with some exceptions. It is evident from the present study that above 50% layer birds are infected with MG organism. Therefore, it may be suggested that the layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically.

Keywords: Seroprevalence, Mycoplasma gallisepticum, serum plate agglutination (SPA), layer birds

Location: Khulna

Start Date: 01-10-2009

End Date: 31-07-2010

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

This paper describes the seroprevalence of MG infection in non-vaccinated layer birds in five upazilas of Khulna district of Bangladesh.

Methodology:

A cross sectional study was conducted on 1,64,120 commercial layer birds of 96 farms in six upazilas (Sadar, Batiaghata, Dumuria, Dighulia, Rupsha and Fultala) of Khulna district during the period from August 2009 to July 2010.A total of 1268 blood samples without any anticoagulant were collected aseptically from wing vein of layer birds using sterile syringe and needle. Sera were separated and stored at -20ºC until used.Standard MG (Nobilis® MG, Intervet International) was used for serum plate agglutination (SPA) test for the detection MG antibodies in the sera samples. The SPA test was conducted according to the manufacturer’s instruction. Briefly, 0.02 ml of antigen and 0.02 ml of chicken serum were placed side by side with micro pipettes on a glass plate. After that antigen and serum sample were mixed thoroughly by stirring with a small tooth pick. Then the glass plate was illuminated from below so as to facilitate observing the reaction, avoiding excessive heat from the light source. Positive reaction was characterized by the formation of definite clumps within 2 minutes after mixing the test serum with antigen. The clumps usually started appearing and became concentrated at the periphery of the mixture. Negative reaction was judged by the absence of agglutination reaction. Care was taken so that no natural granulation of the antigen could be taken as a positive reaction.Chi-square test was done to find out the relationship between seroprevalence of MG infection and independent variables (age of birds, seasons and different areas of study) by using SPSS 11.5

Source of Information: Bangl. J. Vet. Med. (2010). 8(2) : 93–96

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Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The seroprevalence of MG infection was higher in most of the large flocks and lower in small flocks with some exceptions. Higher prevalence in large scale flocks might be due to the faulty in management and biosecurity measures. It is evident from the present study that above 50% layer birds are infected with MG organism. Therefore, it may be suggested that the layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically.

Knowledge Management: