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Qualitative and Quantitative Analysis of Buffalo Ovaries, Follicles and Oocytes in View of in Vitro Production of Embryos

MAMY Khandoker
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh;

N Jahan
Istituto di Zootechnica, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, Piacenza 29122, Italy;

L Asad
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh;

SAM Hoque
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh;

S Ahmed
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh;

MO Faruque
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh;

Abstract/ Executive Summary:

This study was conducted to evaluate the buffalo ovaries, follicles and cumulus-oocyte-complexes (COCs) with the view of in vitro production (IVP) of embryos. Buffalo (Bubalis bubalis) ovaries were collected from local slaughterhouse and categorized according to the position of ovaries in the genital tract (left vs. right) and presence or absence of corpus luteum (CL). Moreover, collected ovaries were also evaluated for length, width and weight, number of follicles, aspirated follicles and state of COCs. The length (cm) of right ovary (2.32±0.06) was significantly (P<0.05) higher than left ones (2.14±0.05). Number of follicles were higher (P<0.05) in the left (7.25±0.31) compare to the right (6.22±0.32) ovaries. Other parameters, including width, weight and number of COC aspirated did not differ significantly (P<0.05) between right and left ovaries. The length, width and weight of ovaries with CL were higher (P<0.05) whereas, number of observed follicles, aspirated follicles, number of COCs and number of normal COCs were significantly (P<0.05) higher in ovaries without CL than their counter parts

Keywords: Buffalo, Corpus luteum, Follicles, Cumulus-oocyte-complexes

Location:

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Buffalo

Objectives:

To collect and evaluate of slaughter house ovaries, follicles and COCs with the view of IVP of embryos.

Methodology:

Buffalo ovaries of unknown reproductive history were collected from local slaughterhouse. The ovaries were kept in collection vial containing 0.9% physiological saline (5 lac iu of penicillin and 100 mg of streptomycin were added per liter of saline on the day of collection) in a thermo flask at 25–30oC and transported to the laboratory within 4-5 hours. The ovaries were then transferred in the sterilized petridishes containing same saline and rinsed thoroughly by physiological solution at room temperature and marked as right and left ovary (right and left ovary was tagged during slaughter with the help of butcher). The presence or absence of corpus luteum (CL) was also recorded.The length and width of ovaries (right and left ovaries; ovaries with CL and ovaries without CL) were measured with the help of a slide calipers and expressed in cm . Weight of individual ovary was measured by placing them on a digital balance and was recorded in a tabular form.

Source of Information: Bang. J. Anim. Sci. 2011. 409 (1-2): 23

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The findings revealed that ovaries without corpus luteum have higher number and higher grade cumulus-oocyte-complexes than those with corpus luteum.

Knowledge Management: Journal