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Optimization of Regeneration Protocol of Rice from Embryo Derived Callus

N. Kamal
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

K. M. Nasuruddin
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. S. Haque
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. Yasmin
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

An efficient and reproducible protocol for in vitro regeneration is required to achieve high frequency transformation from transformed calli. We report here high frequency plant regeneration from mature embryonic calli of two BINA rice cultivars Binasail and Binadhan-4. Embryonic calllus initiated on MS basal medium supplemented with 2 mg/l 2, 4-D. Several media with different combinations of growth regulators were tried. Maximum shoot regeneration frequency (63.33%) was observed in Binadhan-4 on MS medium supplemented with 5 mg/l Kinetin + 0.5 mg/l NAA. Maximum root regeneration frequency (70.00%) was observed in Binadhan-4 on MS medium supplemented with 6 mg/l Kinetin + 0.5 mg/l NAA. Well developed plantlets were hardened and transferred to the glasshouse.

Keywords: Callus culture, Embryo, Regeneration, Rice

Location: The Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh

Start Date: 00-12-2006

End Date: 00-05-2007

Program Area: Variety and Species

Commodity: Rice

Objectives:

To observe the effect of different growth regulators at different concentrations on callus induction and plant regeneration of two BINA varieties.

Methodology:

The experiment was conducted during the period from December, 2006 to May, 2007 at the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh. In the experiment, two BINA rice varieties (Binasail and Binadhan-4) were cultured on MS medium supplemented with different concentrations of growth regulators. At first, sterilized mature seeds attached to endosperm were cultured directly in MS medium supplemented with different combinations of 2, 4-D (0, 1, 2, 3, 4 mg/l) for callus initiation and callus maintenance. Different concentrations of Kn (0, 4, 5, 6, 7, 8, 9 mg/l) with a fixed NAA (0.5mg/l) were used for shoot differentiation and root induction. The experiment was laid out in completely randomized design (CRD). The petrideshs were arranged under 5 replications. The culture petrideshs were placed in growth room, allowed to grow at 25 ± 1°C and data were collected.

Source of Information: Progress. Agric. 18(2) : 25-33, 2007 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Maximum shoot regeneration frequency (63.33%) was observed in Binadhan-4 on MS medium supplemented with 5 mg/l Kinetin + 0.5 mg/l NAA. Maximum root regeneration frequency (70.00%) was observed in Binadhan-4 on MS medium supplemented with 6 mg/l Kinetin + 0.5 mg/l NAA. Well developed plantlets were hardened and transferred to the glasshouse. The results from the present study suggest that, 2 mg/l 2,4-D was the best concentration in Binasail for callus induction and callus maintenance. Binadhan-4 × 5 mg/l Kinetin showed the best shoot regeneration and the highest percentage of root induction was found in Binadhan-4 × 6 mg/l kinetin.

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