Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Bacterial Pathogens and Risk Factors Associated with Mastitis in Black Bengal Goats in Bangladesh

M. A. Islam
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. A. Samad
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

A. K. M. Anisur Rahman
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The objectives of this study were to isolate major bacteria responsible for and to identify the potential risk factors associated with clinical and subclinical mastitis in Black Bengal goats in Bangladesh. A cross sectional study was undertaken on 242 lactating does during January to August 2009. Data on probable risk factors was recorded by using questionnaire. Clinical mastitis was detected by gross signs of udder infection during physical examination and abnormal milk whereas subclinical mastitis was recognized California Mastitis test (CMT) Milk samples of all clinical and subclinical mastitis goats were subjected to culture for isolation and identification of responsible bacterial pathogens. Multiple logistic regression model using a backward stepwise method was used for identification of risk factors. The overall prevalence of clinical mastitis and subclinical mastitis were 4.54% and 37.19%, respectively. Bacterial pathogens isolated were coagulase negative Staphylococcus (73.73%), Staphylococcus aureus (26.67%), Streptococcus sp. (20%), Bacillus sp. (70%) and Escherichia coli (6.67%). Both clinical and subclinical mastitis were significantly associated with age (p<0.001), parity (p<0.001), lactation stage (p<0.001), litter size (p<0.05) and teat lesions (p<0.001).

Keywords: Bacteria, Risk factors, Mastitis, Goat

Location: Mymensingh

Start Date: 01-01-2009

End Date: 31-08-2009

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

This study was undertaken isolate major bacterial pathogens responsible for and to identify potential risk factors of clinical and subclinical mastitis in Black Bengal goats.

Methodology:

The study was conducted on 242 lactating Black Bengal does of three organized goat farms and those brought to Bangladesh Agricultural University Veterinary Clinic during the period from January to August 2009. Clinical mastitis was detected by gross signs of udder infection during physical examination and abnormal milk whereas subclinical mastitis (SCM) was recognized California Mastitis test (CMT). A semi-structured questionnaire was used to collect all related information including husbandry system, age, number of parity, litter size, lactation stage, abnormalities in milk and udder tissues. Ten (10) ml udder half milk samples were collected in labeled sterilized test tubes. Immediately after collection, pH of milk samples was determined using digital pH meter. CMT was performed following the manufacturer’s (Leukocytest®, Synbiotic Corporation, France) instruction and scored as negative (-), weak positive (1+), distinct positive (2+) and strong positive (3+) with method described by Asefa et al. (2006). The isolation and identification of bacterial pathogens were performed according to the procedure described by Quinn et al. (1994). In Brief, 0.1 ml milk sample was cultured on blood agar and nutrient agar. The inoculated plates were incubated at 37°C aerobically for 24-48 hours. The bacterial pathogens were identified by morphology, haemolysis, and biochemical tests like catalse, coagulase and anaerobic fermentation of mannitol (Hargital et al., 1992). Data was entered into Microsoft excels 2007 and transferred to STATA®-12 (Stata corp, USA, 2011) for further analysis. If one quarter milk was CMT positive then that goat was considered as SCM positive. Risk factors were identified using bivariable followed by multivariable logistic regression models (Hosmer and Lemeshow, 1989). Two way interactions among explanatory variables were checked by chi-square test. If there were significant association between two explanatory variables, one of them was excluded from final logistic regression models. Model fitness was assessed by Hosmer-Lemeshow goodness-of-fit test. Significance level s were set as 10% and 5% for bivariable and multivariable models, respective

Source of Information: Bangl. J. Vet. Med. (2011). 9 (2): 155 – 159

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The prevalence CM and SCM was highest in early lactation stage (5.23 and 46.41%) followed by mid (4 and 28%) and late lactation stage (2.56 and 12.82%). Prevalence was also significantly (p<0.001) higher in does with teat lesions (100 and 84.62%) than that of having no teat lesions (0.00 and 14.63%). Teat injury is one of the major clinical sign in mastitic goats observed in this study. It may be concluded from this study that the trend of both clinical and SCM increased with increasing age, number of parity and litter size of does. Prevalence was also higher in early lactation stage.

Knowledge Management: Journal