Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In-vitro Screening of Some Chemicals and Biocontrol Agents Against Erwinia Carotovora Subsp. Carotovora, the Causal Agent of Soft Rot of Potato (solanum Tuberosum)

M. Rashid
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. S. M. Chowdhury
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

N. Sultana
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Abstract/ Executive Summary:

Erwinia carotovora subsp. carotovora was isolated from the infected potato tuber and was identified by pathological, morphological and biochemical studies. Five chemicals viz. Cupravit 50 WP (Copper oxychloride), Sulcox 50 WP (Copper oxychloride), Champion 77 WP (Copper hydroxide), Indofil M- 45 (Mancozeb) and Bavistin 50 WP (Carbendazim) and five biocontrol agents viz. Bacillus subtilis, Pseudomonas fluorescens, Rhizobium leguminosarum, Trichoderma harzianum and Aspergillus flavus were screened out in vitro against the growth of Erwinia carotovora subsp. carotovora by well diffusion method measuring the inhibition zone. Among the chemicals, Sulcox 50 WP (Copper oxychloride) was highly effective against it with 31.00 mm inhibition zone after 48 hours of incubation at 0.2% concentration when 100 μl/well was used. In case of biocontrol agents, Bacillus subtilis was the best against the bacterium with 16.67 mm inhibition zone after 48 hours of incubation.

Keywords: Potato, Soft rot, In - vitro screening, Chemicals, Biocontrol agents

Location: Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka

Start Date: 00-04-2012

End Date: 00-03-2013

Program Area: Pest Management

Commodity: Potato

Objectives:

To identify the causal agent and evaluate the effect of potential antibacterial chemicals and antagonists against it.

Methodology:

The study was conducted at Disease diagnostic laboratory, Department of Plant Pathology, Sher-e- Bangla Agricultural University, Dhaka during April, 2012 to March, 2013. The study included the isolation and identification of the causal organism of soft rot of potato and in vitro screening of some chemicals and biocontrol agents against it. Erwinia carotovora subsp. carotovora was isolated from the infected potato tuber collected from a local market of Dhaka. Potato tuber was surface sterilized with 5% sodium hypochlorite solution for 5 minutes, washed thrice with sterilized distilled water. Sections of lesion margins were placed for 30 minutes in sterile water in a test tube. Tenfold dilution was made from this solution, which was repeated four times to make final dilution up to 10-4. One hundred micro litre of each dilution was spreaded over NA plates at three replications and was incubated at 30±1ºC for 48 hr. Colonies of bacteria were purified on NA plate. A supply of cotton swabs on wooden applicator sticks was prepared following the method. Five selected chemicals viz. Cupravit 50 WP (Copper oxychloride), Sulcox 50 WP (Copper oxychloride), Champion 77 WP (Copper hydroxide), Indofil M-45 (Mancozeb) and Bavistin 50 WP (Carbendazim) were screened against the bacterium by well diffusion method measuring the inhibition zone (Anon, 1996). Four wells of 5 mm in diameter were made in the same NA plate maintaining equal distance and the broth culture of Erwinia carotovora subsp. carotovora was spreaded uniformly on it with sterile cotton swabs. Chemical suspension at definite concentration with different volume was added into the well each at three replications. In case of control, only sterile water was used instead of chemical. The plates were incubated at 30±1ºC. Zone of inhibition around the wells were measured and recorded after every 24 hours for 5 days. Data on different parameters were analyzed by using computer software MSTAT-C and level of significant differences under different treatments and parameters were determined.

Source of Information: The Agriculturists 11(2): 1-9 (2013) ISSN 2304-7321 (Online), ISSN 1729-5211 (Print), A Scientific Journal of Krishi Foundation

Article Link (Online Journal): http://banglajol.info/index.php/AGRIC/article/view/17480/12256

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Erwinia carotovora subsp. carotovora, the causal agent of soft rot of potato was isolated from the infected tuber and was identified. The present findings revealed that among the five selected chemicals Sulcox 50 WP (Copper oxychloride) was highly effective and among the five biocontrol agents, Bacillus subtilis was highly effective against this pathogen in vitro.

Knowledge Management: Journal