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Effects of Protein and Vitamin Ade on Growth Performance and Haemato-biochemical Profile in Broiler

M. Salahuddin
Department of Physiology, Bangladesh Agricultural University, Mymensingh-

M. A. Miah
Department of Physiology, Bangladesh Agricultural University, Mymensingh-

N. Ahmad
Department of Physiology, Bangladesh Agricultural University, Mymensingh-

Abstract/ Executive Summary:

The effects of additional supplementation of protein and vitamin ADE on growth performance and haemato-biochemical profile were studied in "Cobb 500" broiler chicks. The chicks were randomly divided into four equal groups (n=5). Group A was considered as control, fed only with commercial ration and other groups were supplemented with either 20% protein (group B) or 3 ml vitamin ADE/liter D.W. (group C) or both of them (group D) in addition to commercial ration. Body weight was increased significantly (p <0.05) in all three treated groups and highest body weight was recorded in group D. Weights of different organs (liver, skin, legs, breast and viscera) were also varied significantly (p <0.05) among the treated groups. Significant (p < 0.05) differences were observed among the groups for RBC and Hb but increase in PCV values were insignificant (p> 0.05). Serum transaminases (AST, ALT) and creatinine level decreased insignificantly but urea level significantly (p <0.05) varied among the treated groups. The study revealed that combined supplementation of protein and vitamin ADE result better in body weight and different organs weight gain.

Keywords: Haematology, Biochemical profile, Growth performance, Broiler

Location:

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

To study the effects of additional supplementation of protein and Vitamin ADE on growth performances, on hematological parameters such as total erythrocyte count (TEC), hemoglobin (Hb) content, packed cell volume, on cardiohepatic and kidney function.

Methodology:

A total of 20, ten days old “Cobb 500" broiler chicks purchased from Kazi farm limited, were randomly divided into four equal groups (n=5) Group A was considered as untreated control, fed only with commercial ration, group B was supplemented with 20% protein (Jasoprot, protein concentrates, Jason Agrovet Ltd) in feed, group C was supplemented with vitamin ADE ( Acme Laboratories Ltd) (3 ml/liter drinking water ) and group D was supplemented with 20% protein in feed and vitamin ADE (3 ml/liter in drinking water) in addition to commercial ration. Initial body weight of each bird was recorded and kept them group wise. Fresh water was supplied to the broilers ad libitum. Feed supplementations were prepared on daily basis.Managements and rearing of birds were strictly followed according to standard broiler farming system. In order to prevent stress, shock, deficiencies and infections broiler chicks were routinely immunized and antibiotic premix were used as per recommendation of manufacturer. Proper hygienic and strict sanitary measures were also taken during the experimental periods. Blood from each bird was collected at slaughter. A number of sterile test tubes containing anticoagulant (3.8% Trisodium citrate solution) at a ratio of 1:10 were taken. The hematological studies were performed within two hours of collection. Sera were prepared for biochemical (Creatinine, AST, ALT, and Urea) analysis according to standard procedures. Briefly, 3 ml of blood was collected in the sterile glass test tube. The blood containing tubes were placed in a slanting position at room temperature for clotting. Then the tubes were incubated overnight in the refrigerator (4°C). Then serum was collected. The sample was centrifuged at 1000 rpm for 15 minutes to have a more clear serum. The serum samples were separated and stored at -20°C till analysis. Serum creatinine, transaminases and urea were assayed by conventional enzymatic methods on a Hitachi 911 automated analyzer from Roche Diagnostics (Laval, QC, Canada) according to the manufacturer’s specifications All data were expressed as mean ± standard error and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncan’s multiple range test. Statistical analysis was performed using SPSS software

Source of Information: Bangl. J. Vet. Med. (2012). 10 (1&2): 9–14

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among the serum bio-chemical parameters, creatinine, aspertate aminotransferase (AST) and alanine transaminase (ALT) values were found insignificantly lower in all treated groups compare to control group . The urea level in treated group D receiving protein and vitamin ADE was found to be significantly higher than others. Vitamin ADE could not alter the urea level greatly. Urea level of group B was apparently low compared to control group A.

Knowledge Management: Journal