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Growth Regulatory Activities of Different Extracts of Tinospora Cordifolia in Some Vegetable Seeds with Their Chemical Investigation

S. Aktar
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur

M. A. Sayed
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur

M. R. Islam
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur

B. Roy
Department of Agricultural ChemistryHajee Mohammad Danesh Science and Technology University, Dinajpur

M. A. Hossain
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur

Abstract/ Executive Summary:

An experiment was conducted to investigate the growth regulatory activities of different extracts of Tinospora cordifolia on radish (Raphanus sativus), swamp cabbage (Impoea aquatica) and lady’s finger (Hibiscus esculentus) with the attempt for chemical investigation of effective plant extract. The chloroform extract of Tinospora cordifolia significantly increased and enhanced germination, growth of shoot length and root length of radish and lady’s finger whereas and delayed germination, growth of shoot length and root length of swamp cabbage seeds compared with control. In the same way, ethanol extract of Tinospora cordifolia significantly increased germination, growth of shoot length and root length of swamp cabbage followed by control and chloroform extract. Our study reveals that different extracts of Tinospora cordifolia contain growth regulatory active principle. Among the extracts, chloroform extract was shown better performance in terms of percent germination, growth of shoot and root length of radish and lady’s finger. To find out effective compound from chloroform extract, thin layer chromatography was done and showed five distinct compounds. Further study is needed to determine structure for finding growth regulatory compound for agricultural usage.

Keywords: Chemical investigation, Extract, Growth regulatory activity, Vegetables

Location: Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

Start Date: 00-01-2011

End Date: 00-12-2011

Program Area: Food Safety and Security

Commodity: Cabbage

Objectives:

1. To investigate the growth regulatory effects of different extract of T. cordifolia and isolate the different bioactive compounds from the effective extract of T. cordifolia.

Methodology:

Experimental Site - The experiment was conducted at research laboratory, Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh, during January 2011- December 2011. Collection of T. cordifolia and summer vegetable seeds - T. cordifolia stems were collected from Chalksudum of Chirirbandar under Dinajpur district. Radish (Raphanus sativus), Swamp cabbage (Impoea aquatic) and Lady’s finger (Hibiscus esculentus) seeds were selected due to their short growth period and available in sub-tropical countries. The seeds of these vegetables were collected from the Dinajpur seed market. The purity percentages and germination percentages of these seeds were 95 and 90, respectively. Preparation of aqueous extracts of T. cordifolia - About 50 gm of fresh stems of T. cordifolia were cut into smaller pieces and blended by blender and taken into a 1000 ml reagent bottle with water up to the mark. It was kept for 72 hours at room temperature with regular interval of stirring. After 72 hours the resulting brownish and dark solution were filtered through three layers of Whatman No. 1 filter paper and was taken into another 1000 ml bottle. The filtrates of plant extract were stored and used for treating the seeds of vegetable crops. Preparation of chloroform extract and ethanol extract of T. cordifolia - Ten kilogram of green and fresh stem were cleaned and cut into small pieces. The small pieces of stem were dried in sun light for 7 days followed by oven at 70^o C for 48 hours. The dried stem was grinded by grinding machine and obtained about 4 kg powder. Two kilogram powder was dissolved in five liter absolute chloroform (96%) and another two kilogram in absolute ethanol (98%) and incubated for 72 hours for a suspension. These suspensions were filtered with thin and clean cloth and finally filtered by filter paper. The suspension was dried by BUCHI Rota vapor R-114 connected with BUCHI water bath B-480 at 70^oC. The dried extract was weighed by digital balance. Five percent solution of each extract was prepared with water for treating the seeds of vegetable crops. The individual plant extracts were investigated as following sequential treatments: a) Water or control (Tc) b) Aqueous extract of T. cordifolia (T₁) c) Chloroform extract of T. cordifolia (T₂) d) Ethanol extract of T. cordifolia (T₃). Set up for the investigation of vegetable crop seeds - Thirty six petridish were cleaned and divided into three groups for three vegetables seeds and two sheets filter papers were placed on it. For each vegetable seeds, petridish were divided into four treatments with three replications each petridish separately. 15 ml of each aqueous, chloroform, ethanol extract and distilled water put in each petridish according to experimental design. After that 25 seeds of each vegetable crop were kept in each petridish. The petridishes were placed in natural diffused light under laboratory conditions at 29±2°C temperature and relative humidity of 85±5%. Five ml of water was applied per day per petridish to keep constant moisture. The percent germination, shoot length, root length and completion of germination were recorded and analyzed statistically using Duncan’s Multiple Range Test (DMRT). After analyses these data, it was observed that the chloroform extract of T. cordifolia enhance the germination, shoot length and root length of radish and Lady’s finger and inhibit germination, shoot length and root length of swamp cabbage compared with control.

Source of Information: J. Environ. Sci. & Natural Resources, 5(1): 133 - 140, 2012; ISSN 1999-7361

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The chloroform extract of Tinospora cordifolia significantly increased and enhanced germination, growth of shoot length and root length of radish and lady’s finger whereas and delayed germination, growth of shoot length and root length of swamp cabbage seeds compared with control. In the same way, ethanol extract of Tinospora cordifolia significantly increased germination, growth of shoot length and root length of swamp cabbage followed by control and chloroform extract. Our study reveals that different extracts of Tinospora cordifolia contain growth regulatory active principle. Among the extracts, chloroform extract was shown better performance in terms of percent germination, growth of shoot and root length of radish and lady’s finger.

Knowledge Management: Journal