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Frequency and Distribution of Immunoglobulin Containing Plasma Cells in Ileum Representing Gut-associated-lymphatic Tissues in the Bcrdv-vaccinated Broiler

M. A. Masum
Department of Anatomy, Histology and Physiology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University

M. Z. I. Khan
Faculty of Veterinary Medicine, University Malaysia Kelantan, Locked Bag 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.

M. N. H. Siddiqi
Department of Anatomy and Histology, Faculty of veterinary Science, Bangladesh Agricultural University, Mymensingh

M. Nasrin
Department of Anatomy and Histology, Faculty of veterinary Science, Bangladesh Agricultural University, Mymensingh

N. Sultana
Department of Anatomy and Histology, Faculty of veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

A total 24 day-old "Cob-500" broiler chickens of both sexes were purchased from "Kazi Farm Ltd." Mymensingh. The chickens were divided into two groups such as vaccinated group (each of 12 broilers) and control group (each of 12 broilers). First, second and third sample was collected from both vaccinated and control group at Day 1 (D₁), Day14 (D₁₄) and Day 28 (D₂₈) respectively. In the present study, histological examination revealed that the ileum was a tubular structure of the gastrointestinal tract situated in between jejunum and cecum. The lining cells of the villi of the ileum of chickens were simple columnar epithelium. The villi of the ileum were long and slender; numerous goblet cells were present among the lining epithelial cells. Histologically, the scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. Lymphocytes in the lamina propria and intraepithelial lymphocytic distribution were increased in concentration in vaccinated group. The length of mucosa of ileum at cells at D₁_, D₁₄ and D₂₈were 154±0.682 Lm, 560±0.682 Lm and 1020±0.725 Lm, respectively. The width of mucosa of ileum at cells at D₁, D₁₄ and D₂₈ were 28±0.025 Lm, 107±0.625 Lm and 140±0.365 Lm, respectively. The length and width of mucosa is highest at D₂₈. In this regards, there is no available data. It may be due to aging of broiler

Keywords: Broiler, Immunoglobulins, Vaccine

Location: Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

To develop and evaluate vaccines and to optimize vaccine application.

Methodology:

The experiment was carried out in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. A total 24 day-old "Cob-500" broiler chickens of both sexes were purchased from "Kazi Farm Ltd." Mymensingh. The chickens were divided into two groups such as vaccinated group (each of 12 broilers) and control group (each of 12 broilers). The chickens had no developmental disorders and detectable diseases that may influence in the distribution of lymphocytes and plasma cells containing different classes of immunoglobulins (IgA, IgG and IgM) in ileum which represent gutassociated- lymphoid tissues of broiler. The chickens were reared in a litter system in a poultry shed of the Department of Poultry Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh. Bio-security of the poultry shed was maintained strictly and brooding management was also strictly followed. Optimum temperature, lighting and ventilation were maintained in the brooder. At day 3, BCRDV vaccine was administered through intraocular route for the prevention of Baby Chick Ranikhet Disease. For histological and immunohistochemical staining purpose, ileum was collected, after sacrificing the chickens through cervical subluxation method. First, second and third sample was collected from both vaccinated and control group at Day 1 (D₁), Day14 (D₁₄) and Day 28 (D₂₈) respectively. For histological studies, the samples were collected, cut into pieces, and then fixed in the "Bouin's fluid" (Gridley, 1960) for 12 hours. The selected samples were dehydrated in a series of ascending grades of alcohol (70%, 80%, 95% and 100%), cleared in several changes of xylene, and infiltrated with different grades of melted paraffin in the oven at 52°C. The tissues were then embedded in liquid paraffin and finally the sections were cut at 6Lm thickness using sliding microtome (MIC 509, Euromex, Japan). After sectioning, the sections were floated on luke warm water in a floatation bath at 37°C for stretching, then the sections were attached on cleaned glass slides using egg albumin and then dried. The sections were then deparaffinized first in several changes of xylene followed by rehydration in a series of descending grades of alcohol (100%, 95%, 80% and 70%). The sections were dipped in Hematoxylin (basophilic dye) for 10-15 minutes (as required) and Eosin (acidophilic dye) for 2-4 minutes (as required) (Gridley, 1960). After staining, the sections were dehydrated again in a series of ascending grades of alcohol (70%, 80%, 95%, and 100%), cleared in xylene and mounted with "DPX".

Source of Information: Bangl. J. Vet. Med. (2012). 10 (1&2): 15-20

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, histological examination revealed that the ileum was a tubular structure of the gastrointestinal tract situated in between jejunum and cecum. The lining cells of the villi of the ileum of chickens were simple columnar epithelium. The villi of the ileum were long and slender; numerous goblet cells were present among the lining epithelial cells. Histologically, the scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. Lymphocytes in the lamina propria and intraepithelial lymphocytic distribution were increased in concentration in vaccinated group. The length of mucosa of ileum at cells at D₁_, D₁₄ and D₂₈were 154±0.682 Lm, 560±0.682 Lm and 1020±0.725 Lm, respectively. The width of mucosa of ileum at cells at D₁, D₁₄ and D₂₈ were 28±0.025 Lm, 107±0.625 Lm and 140±0.365 Lm, respectively. The length and width of mucosa is highest at D₂₈. In this regards, there is no available data. It may be due to aging of broiler

Knowledge Management: Journal