Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Investigation on the Efficacy of Salmonella Gallinarum Killed Vaccine

R. Khatun
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

M. A. Parvez
Department of Medicine and Surgery, Chittagong Veterinary and Animal Sciences University, Chittagong

M. G. Hossain
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

S. Saha
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

M. M. Amin
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The study was undertaken to investigate the efficacy of Salmonella gallinarum vaccine prepared at the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh. The vaccine induced immune response both in chicken and mice are determined by PHA antibody titre and protection test. The highest PHA antibody titer was at 15 days after booster vaccination in both chickens and mice. The chickens and mice of vaccinated groups conferred 100% protection following challenge infection with the virulent isolate of Salmonella gallinarum given at 2 weeks after final immunization through i/m route (p <0.01). Differential leukocyte count (DLC) was performed in vaccinated mice and it was revealed that 11% increase in lymphocyte count in vaccinated group compared to control group (p <0.01). Finally, passive protection test in chickens that the protective value in terms of overall survival rate was 100% (p <0.001). These results clearly demonstrated that the Salmonella gallinarum vaccine of LPVRPC induced satisfactory level of antibody titre.

Keywords: Salmonella gallinarum, efficacy, vaccination, PHA test and protection

Location: Mymensingh

Start Date: 01-07-2011

End Date: 31-12-2011

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

The present research was undertaken in Shaver brown chicken to investigate the immunogenicity and protective activity of Salmonella gallinarum vaccine with mechanism of protection produced by LPVRPC, Department of Microbiology and Hygiene, Bangladesh Agricultural University.

Methodology:

The research was conducted during the period of July 2011 to December 2011 in the Bacteriology laboratory of the Department of Microbiology and Hygiene, BAU, Mymensingh. A total of 70 mice (Balb/c) were obtained from the experimental animal shed of Department of Microbiology and Hygiene, BAU, Mymensingh and used for safety, efficacy test and determination of LD₅₀ dose. Twenty (20) eight-week-old layer birds (Shaver brown strain) were obtained with the courtesy of Phenix Poultry Hatchery Limited. Another twenty (20) Hisex white chickens of eight-week-old were taken and used for passive protection test. The mice and birds were provided with balanced diet, pure water, and other managemental requirements for proper bio-security. Five blood agar (BA) plates were inoculated with five Salmonella gallinarum vaccine and incubated at 37⁰C for 24- 48 hours in the incubator for the growth of aerobic and anaerobic organism. The Salmonella gallinarum vaccine which did not exhibit the growth of aerobic and anaerobic organism was used in the experiment (Heddleston and Reisinger, 1960). The safety test was carried out following the method of Matsumato and Helfer (1977) and Dorsey (1963). Five mice were inoculated subcutaneously (S/C) with 0.5 ml of each vaccine and the vaccine considered safe if the inoculated mice remained alive and healthy during the observation period of 5 days. Determination of LD₅₀ dose in mice Salmonella gallinarum was cultured in nutrient broth overnight at 37⁰C in a shaker incubator. After 12 hours growth, centrifuged at 1500 rpm for 5 minutes. Then the supernatant was decanted off and bacterial mass was re-suspended in PBS by shaking. This process was repeated three times. The pellet was then dissolved in sufficient amount of PBS and OD value was measured at 550 nm and OD value was adjusted at 2.8. A 250 Jl, 100Jl, 50Jl and 25 Jl bacterial suspension or PBS were injected intra-peritonially in mice (n=10) of each group. Death pattern was observed for subsequent 10 days. A 10-fold dilution of the bacterial suspension was made and spread on nutrient agar (NA) media for counting of CFU (8.6×1013 CFU/ ml). A 25 Jl of 8.6×1013 CFU/ml contain LD₅₀ dose in mice.Statistical analysis of PHA antibody titer was performed using Student’s paired t-test (p ≤ 0.05 was considered statistically significant). Differences in leukocyte count were analyzed by Mann-Whitney test (p ≤ 0.01 was considered statistically significant). Challenge infection and passive protection test were analyzed by Mantle-Cox Log rank test (p ≤ 0.01 was considered statistically significant).

Source of Information: Bangl. J. Vet. Med. (2012). 10 (1&2): 41-49

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Salmonella gallinarum vaccine produces higher level of antibodies both in chicken and mice as revealed by PHA test (p <0.05). This study revealed that an 11% increase in the lymphocyte count in vaccinated mice than in the control group (p <0.01).Vaccinated chickens protected from lethal challenge (p <0.01) and the passive serum treatment had a definite protective value in terms of overall survival indicating Salmonella Gallinarum vaccine induce passive protection immunity through humoral immune response (p <0.01).

Knowledge Management: Journal