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Effect of Rhizobium Strains and Biofertilizers on Foot, Root Rot and Yield of Bush Bean in Sclerotinia Sclerotiorum Infested Soil

K M Khalequzzaman
Plant Pathology Division, Regional Agricultural Research Station, BARI, Ishurdi, Pabna, Bangladesh

I Hossain
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Abstract/ Executive Summary:

A glasshouse experiment was carried out to find out the effect of Rhizobium strains and biofertilizers on foot and root rot (Sclerotinia sclerotiorum) and yield of Bush bean. Six Rhizobium strains and three biofertilizers were used for seed treatment. Rhizobium strains (BINAR P36 and BINAR P6) and BINA biofertilizer resulted maximum reduction of seed rot, and foot and root rot of Bush bean in pot. In addition, these treatments increased germination, plant stand, shoot length/plant, root length/plant, shoot weight/plant, root weight/plant, vigour index, plant height, number of green pods/plant, weight of green pods/plant, weight of seeds/plant and healthy looking seeds in pot trials. Among them Rhizobium strains (BINAR P36) showed best performance.

Keywords: Rhizobium, Biofertilizer, Foot and root rot, Sclerotinia sclerotiorum, Yield, Bush bean.

Location: Glasshouse of the Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh

Start Date: 00-00-2002

End Date: 00-00-2003

Program Area: Crop-Soil-Water Management

Commodity: Bush bean

Objectives:

The present study was undertaken to achieve the effect of Rhizobium strains and biofertilizers in controlling the foot and root rot (Sclerotonia sclerotiorum) and on yield of Bush bean under glasshouse condition.

Methodology:

The experiment was laid out at glasshouse of the Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh during 2002–2003. The variety was used BARI Bush bean 1.Inoculum of Sclerotonia sclerotiorum was prepared following the method of Khan (1998). The soil was mixed uniformly with cow dung (2:1). The dried soil was sterilized with formalin (40%) at the rate of 5 ml formalin diluted with 20 ml of water for 4 kg soil (Dasgupta 1988, Hossain 2000). The oat culture of Sclerotium rolfsii was then mixed with pot soil at the rate of 0.1% weight of dry soil before seed sowing (Khan et al 1998, Hossain et al. 1999, Hossain 2000, Kibria and Hossain 2002). The inoculum was applied only up to a depth of 6 cm in the pot soils i.e. 2 g of oat culture was mixed with each pot soil. A total of 50 pots were inoculated with the oat culture for five replications. Seeds were counted, weighed and packed in separate polyethylene bags as per treatment and replication. Six Rhizobium strains (BAUR 424, BAUR 979, BARIR 1000, BARIR 7029, BINAR P6 and BINAR P36) and three biofertilizers (BAU biofertilizer, BARI biofertilizer and BINA biofertilizer) were used for treating seeds. Composition of biofertilizers was peat soil and Rhizobium strains. Seeds were treated with Rhizobium strains and biofertilizers following the method of Somasegaran and Hoben 1994 and Sattar 1997. For seed coating with Rhizobium broth, broth containing 109 Rhizobium cells/ml was taken in sterilized 500 ml Erlenmeyer flasks. The seeds were dipped in broth for 15 minutes. In case of biofertilizers, seeds were initially moistened with molasses at the rate of 50 g per kg of seeds. Then the seeds were thoroughly mixed with biofertilizer (50 g/kg of seed), where each biofertilzer contained 108 Rhizobium cells/mg. In case of control, seeds were not treated with Rhizobium strains or biofertilizers. The inoculant-coated seeds were placed in a cool and dry place under shade for drying. The Rhizobium treated seeds and non-treated seeds for control were sown in previously prepared soil in pots. Five seeds were sown in each pot. The pots were inspected at every day in the morning to take observations on foot and root rot diseased plants. Intercultural operations were done to maintain the normal hygienic condition of crop growth in pots. No plant protecting chemicals (insecticides or fungicides) were used for controlling the pests and diseases of the crop. The data were recorded on germination (%), seed rot (%), foot and root rot (%), plant stand (%), seedling vigour, number of nodules/plant, length of green pod (cm), breadth of green pod (cm), no. of green pods/plant, wt. of green pods/plant (g), plant height (cm), no. of seeds/pod, wt. of seeds pod (g), wt. of seeds/plant (g), apparently healthy looking seeds (%) and discoloured seeds (%). For vigour index (VI) or seedling vigour, data were recorded on germination up to 15 days of sowing. Five plants were randomly selected and uprooted carefully from pot and washed in tap water. Then root length and shoot length were recorded. Vigour index (VI) was calculated by using the formula of Baki and Anderson (1973). Recorded data were subjected to statistical analysis for mean values and test of significance. The variations among the respective data were compared following the Duncan’s New Multiple Range Test (Gomez and Gomez 1984).

Source of Information: J. bio-sci. 16: 73-78, 2008 ISSN 1023-8654

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Overall results of the present study indicate that among the Rhizobium strains and biofertilizers, BINAR P36, BINAR P6 and BINA biofertilizer resulted maximum reduction of seed rot, and foot and root rot and increased yield and yield attributes of Bush bean. BINAR P36 showed best performance.

Knowledge Management: Journal