Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Differential Response of Potato Under Sodium Chloride Stress Conditions in Vitro

M H Rahman
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

R Islam
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

M Hossain
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

S A Haider
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

In vitro evaluations of salinity (NaCI) effects on three potato cultivars (Atlanta, Shepody and Shilbilaty ) were investigated with five NaCI levels ( 0, 25, 50, 75 and 100 mM) by using single node explants. Significant differences were noticed among the cultivars followed by different NaCl levels. Salinity stress gradually depressed plant growth and root development with increased NaCl concentration in MS media. All the cultivars survived at high NaCl (100 mM) containing MS media with exhibiting different growth status. The results indicate that Shilbilaty performed better in shoot length and shoot fresh mass than Shepody and Atlanta. The Atlanta performed better in root growth than Shepody and Shilbilaty at different NaCl media. Highest salinity level drastically inhibits root growth in all the cultivars tested. The control and 25 mM NaCl containing MS media did not affect the growth traits of in vitro potato plantlets. The control was found superior in growth characterized than rest of the tested NaCl levels.

Keywords: Potato, Single node, NaCl stress, Growth response

Location: Department of Botany, University of Rajshahi

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Potato

Objectives:

The objective of this study was to find out the effect of different levels of NaCl on in vitro growth traits of three potato cultivars by using single-node cuttings and to evaluate salt-tolerant cultivars effective for future use under salinity conditions.

Methodology:

Single-node cutting bioassay: Single node cuttings of potato (Solanum tuberosum L.) plantlets derived from meristem culture were subjected to initiate this experiment. Four week grown in vitro plantlets of Atlanta, Shepody and Shilbilaty (Bangladeshi Indigenous cultivar) were prepared with shoot apex and root discarded and single nodes (1cm) were separated and cultured in MS medium having 3% sucrose, 0.8% agar with a range of NaCl (0, 25, 50 75 and 100 mM) treatments. Potato plantlets grown in high NaCl (100 mM) media were recultured in the same concentration of NaCl media and NaCl-free MS (Murashige and Skoog 1962) media to check their growth consistence and their tolerance potential in vitro. All the cultures were incubated at 25±2ºC with 16/8 h D/N cycle at 15μmol m -2 s -1 photon flux density (cool white fluorescent light). The pH of the medium was adjusted to 5.8 and autoclaved at 121ºC for 20 min. Three nodal segments were implanted in each culture vessel and four replications of each genotype at each salinity level were employed. The experiment ended on 4 weeks and the growth response were noted on shoot length (SL), root length (RL), shoot fresh mass (SF) and root fresh mass (RF). Growth data were evaluated at each salinity level with control (no NaCl trace). The data were analyzed by IRRISTAT program and the differences among the treatments were tested with least square difference (LSD) at 5% level of significance.

Source of Information: J. bio-sci. 16: 79-83, 2008, ISSN 1023-8654

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

We recommend taking four growth parameter measurements at 0, 25, 50, 75, and 100 mM NaCl for further micropropagation scheme and consequently it can be used as a source of salt-tolerant line for field transplantation where salinity level is a major obstacle to potato production.

Knowledge Management: Journal