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In Vitro Androgenesis of Some Wheat (triticum Aestivum L.) Varieties and Their F1, F2 Progenies and Combining Ability of Embryoid Production

M. Hasan
Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706, Bangladesh

J. Pauk
Biotechnology Division, Cereal Research Non-Profit Organization, H-6701, P.O. Box 391, Hungary

Z. Kertesz
Wheat Breeding Division, Cereal Research Non-Profit Organization, H-6701, P.O. Box 391, Hungary

Abstract/ Executive Summary:

Six parental lines along with their F₁ and F₂ progenies were investigated for general combining ability (GCA) effects on embryo-like-structure (ELS) production in anther culture. Significant differences were found among the parents in respect to the ELS production. Evaluating the combining ability effects, among the female parents Alfold showed the best result in ELS production, while Palma was the best among the male lines. While comparing F₁ and F₂ generations, in most of the cases (except one) F₂ generation was superior in ELS production. Polygenic effect plays a key role in such type of superiority in the F₂ generation. The reason for such superiority may be due to the recombination of superior genes.

Keywords: Anther culture, Combining ability, ELS

Location: Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Wheat

Objectives:

To estimate combining ability of some cultivated Hungarian wheat varieties for microspore embryoid production and also to check the embryoid production responses of their F₁ and F₂progenies.

Methodology:

Six parents and their nine F₁and F₂ generations were used for androgenic wheat studies. The donor plants were grown in a greenhouse under standard growing conditions. Cold treatment of donor shoots for anther culture was carried out in a cool chamber (4⁰C) for 14-16 days. Spikes containing early and mid-uninucleate stage microspores were surface sterilized with 2% NaOCl containing some drops of Tween-80 for 20 minutes and then rinsed with sterile distilled water. Excised anthers (150 each in 5 replications) were plated onto a modified P-4 induction medium in 60 mm plastic Petri dishes. The P-4 medium was improved in two important components. Instead of sucrose and agar, 8% maltose and 10% Ficoll were used, respectively. For plant regeneration, a modified 190-2 medium was applied (190-2 Cu) increasing CuSO₄ x 7H₂O doses twenty times compared to the content in the original MS medium. Floated anthers in Petri dishes were kept in a dark room at 32^oC for 3 days and after that at 28⁰C for embryoid induction. ELS of about 1.5-2 mm were transferred to regeneration medium. Parents and progenies embryo-like-structure (ELS) production was analyzed by Duncan’s Multiple Range Test.

Source of Information: Progress. Agric. 18(2) : 57-60, 2007 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Six parents and their 9 F₁ and F₂ generations were tested regarding their androgenic response. Significant differences were found in anther culture response among the tested varieties. When evaluating the combining ability effects for ELS production, among the female lines Alfold was the best, while among the male lines Palma had best performance. When F₁ and F₂ generations were compared, F₂ had better performance. It might be due to polygenic effect in the F₂ generation. The reason for such superiority may be due to the recombination of superior genes. Based upon all the results obtained from the experiments, it is advisable to cross wheat parents with good combining ability.

Knowledge Management: Journal