Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Propagation of Thuja Occidentalis Through Apical Shoot Culture

M. H. Kabir
Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, G.P.O. Box No. 3787, Dhaka

P. K. Roy
Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, G.P.O. Box No. 3787, Dhaka

Golam Ahmed
Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, G.P.O. Box No. 3787, Dhaka

Abstract/ Executive Summary:

In vitro plant regeneration of Thuja occidentalis was obtained in apical shoot cultures from field grown plants. Hormone free MS medium 100 % explants produced shoots. The average number of shoots per explant was 6.57 ± 0.45 and the average shoot length of 4.5 ± 0.27 cm were recorded in this medium. Shoots rooted well when they were transferred into half strength MS with 1.0 mg/l IBA. The average number of root per shoot was 3.92 ± 0.28 and the average root length of 3.64 ± 0.38 cm were observed in this medium. No morphological variants were observed during the passage of in vitro culure.

Keywords: In vitro, Propagation, Thuja occidentalis, Apical shoot

Location: Savar, Dhaka

Start Date:

End Date:

Program Area: Resource Development and Management

Commodity:

Objectives:

The present study was conducted to establish a reproducible protocol for large scale in vitro production of Thuja occidentalis using apical shoots as explants.

Methodology:

Apical shoots of field grown Thuja occidentalis were collected from AERE Campus, Savar, Dhaka. The explants were washed thoroughly using household detergent, Trix for 15 minutes under running tap water. Subsequent sterilization was carried out in the laminar air flow cabinet under aseptic conditions. Shoots were sterilized in 40% Clorox for 5 minutes followed by 0.1% mercuric chloride for 5 minutes. Rinsing was done three times with sterile distilled water. Apical shoots of about 2 cm long were excised and inoculated into media containing half strength MS, MS0 and MS media supplemented with different concentrations of BA and Kn alone or BA in combination with NAA for shoot induction. Subculture was done 30 days interval on the same medium for promoting strong and healthy multiple shoots. Morphologically healthy shoots were transferred to half strength MS supplemented with different concentrations of IBA, IAA and NAA for root induction. The sucrose concentration was used 30 g/l and the pH of the media adjusted to 5.8 prior to autoclaving. Cultures were incubated at 26 ± 2°C with a 16 hour illumination of 21.8 μmol/cm²/s provided by cool white fluorescent tubes. The experiment was conducted with 30 explants per medium component. Data were collected on different characters at day 90 for multiple shooting and at day 60 for rooting of shoots. Observation on cultures were carried out daily.

Source of Information: Plant Tissue Cult. & Biotech. 16(1): 5-9, 2006 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The rooting responses differed among the auxins used. IBA was found responsive for root induction and 1 mg/l IBA was found optimum, in which 100% shoot rooted within 15 days of culture. The average number of roots per shoot was 3.92 ± 0.28 and the average root length of 3.84 ± 0.38 cm were observed in this medium. Shoots did not respond for rooting in the media containing IAA or NAA and they eventually died. Morphologically strong and healthy rooted about 5.0 cm tall shoots were taken out from the culture vessels and washed gently under running tap water to get rid of agar. The in vitro raised plantlets were then transferred to a small earthen pot containing a mixture of soil and compost (2 : 1) and acclimated following the usual procedures shade and mixed twice a day. About 60% plantlets resumed new growth within 30 days of acclimation period. The study showed that it is possible to successfully regenerate complete plantlets from apical shoots of filed grown plants of Thuja occidentalis. Thus this protocol might be used for Thuja propagation commercially.

Knowledge Management: Journal