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Effects of Liquid Medium on Rooting and Acclimation of Regenerated Microshoots of Banana (musa Sapientum L.) Cv. Sagar

M. Atique Akbar
Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Shyamal K. Roy
Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

A regeneration protocol ensuring a high frequency rooting of micro-shoots derived from apical and auxiliary buds of suckers of banana cv. Sagar was achieved by using liquid medium. When the explants were cultured on MS medium supplemented with 0.5 mg/l each of BA, Kn and NAA, a large number of shoots developed. With the progression of the number of subcultures, shoot proliferation was enhanced. Addition of 10 % coconut water to the medium increased the number of shoots per culture and growth of individual shoots. Shoots rooted well within two weeks, when they were separated individually and sub-cultured in 1.0 mg/l IBA supplemented half strength MS liquid and agar-gelled medium but rooting percentage and their growth were superior in the liquid medium to agar-gelled medium. Rooted plantlets were transferred to small polythene bags containing sterile sand, soil and humus (1 : 2 : 1) and maintained with a high humidity for acclimation. Acclimation and transplantation performances were found to be superior in plants that were rooted in the liquid medium.

Keywords: Liquid medium, Regeneration, Transplantation

Location: Savar, Dhaka

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Banana

Objectives:

This paper reports results of studies of an important parameter, namely a high rate of acclimation, using liquid medium in inducing rooting in in vitro regenerated local banana cultivars.

Methodology:

Four-six weeks old suckers were collected from healthy plants growing in cultivated fields in Kaliganja Upajila of Jhenaidah district. The suckers were brought to the laboratory and washed thoroughly under running tap water. Outer leaves with leaf-sheaths of the suckers were peeled off until the size became to 5-6´ × 2-3 cm. Surface sterilization was carried out with 70% (v/v) alcohol for 15 min, after which they were rinsed three times with sterile water in a laminar air-flow cabinet. The plant materials were then dipped in 0.2 % HgCl₂solution for ten min. followed by washing three times with sterile distilled water to wash out any trace of HgCl₂. After surface sterilization these were trimmed 2 - 3 mm from both ends and the outer sheath was peeled off. The plant materials were then longitudinally dissected into two unequal halves of which one half contained apical and auxiliary shoot buds and the other half contained only auxiliary shoot buds. The individual explants were cultured in agar gelled (0.7%) MS nutrient medium containing different concentrations and combinations of cytokinins, auxins and CW. For rooting, half strength MS supplemented with auxin was used individually in two supporting materials, one with 0.7% agar and another in liquid substratum. Three per cent sucrose was used in all media preparations. The media were adjusted to pH 5.8, and autoclaved for 20 min at 121⁰C under 1.1 kg/cm² pressure. Cultures were incubated at 25 ± 1⁰C under 16 h photoperiod with a light intensity of 3000 - 4000 lux.

Source of Information: Plant Tissue Cult. & Biotech. 16(1): 11-18, 2006 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It was observed that the survival rate of the plants, which were rooted in liquid medium was higher. When the rooted plantlets were taken out from the rooting medium and washed, injury of root system generally occurred in case of agar-gelled medium, whereas in liquid medium no such injury was observed. Lack of injury during transplantation of plantlets was probably the cause of higher rate of survival. Moreover, use of liquid medium for root induction in rooting was found to be highly economical. The protocol described here is promising for the large scale production of disease free planting materials of the Sagar variety of banana.

Knowledge Management: Journal