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Analysis of F2 Population of Intraspecific Cc Genome Oryza by Using Ssr Marker

M. Shalim Uddin
Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh

Shamim Ara Bagum
Seed Technology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

D. A. Vaughan
Crop Evoluation Dynamics Team, National Institute of Agrobiological Science, Tsukuba,Japan

P. L. Sanchez
Crop Evoluation Dynamics Team, National Institute of Agrobiological Science, Tsukuba,Japan

N. Tomooka
Crop Evoluation Dynamics Team, National Institute of Agrobiological Science, Tsukuba,Japan

Abstract/ Executive Summary:

Of the 22 Oryza species, three are diploid CC genome species and six are allotetraploid species sharing the CC genome. The diploid CC genome species have useful genes such as those conferring resistance to brown plant hopper, white back plant hopper, bacterial blight and yellow dwarf virus disease. This work describes SSR marker analysis of progeny derived from crosses in O. eichingeri (CC genome). Twelve Microsatellites or Simple Sequence Repeats (SSR) markers have been used to analyze 82 F₂ progeny plants from an intraspecific cross, O. eichingeri (Uganda) × O. eichingeri (Sri Lanka). The markers were distributed on 8 of the 12 linkage groups. Most of the SSR markers had the expected distribution of plants segregating for the parental or heterozygous condition except for RM109 which was present in more individuals with the genotype of the parent from Sri Lanka than the heterozygous or Uganda parent. The F₂ population showed transgressive segregation for days to heading. Some characters had a normal distribution such as internode color and leaf size. Other characters showed segregation toward one of the parents viz. panicle type toward the Uganda parent and auricle color and leaf morphology toward the Sri Lankan parent.

Keywords: SSR marker, F2 population, Intraspecific hybrid, CC genome, Oryza

Location: Crop Evoluation Dynamics Team, National Institute of Agrobiological Science, Tsukuba,Japan

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

The present investigation was carried out for analysis of F₂ population of intraspecific CC genome Oryza by using SSR primer which will be helpful for gene mapping of CC genome of Oryza.

Methodology:

Two accessions of O. eichingeri Peter (W1519 from Uganda and SL6 from Sri Lanka) were selected to develop a CC genome mapping population. The hybridbetween these accessions was produced at the National Institute of Agrobiological Science (NIAS), Tsukuba, Japan. A single crossed hybrid was selfed and F₂ seeds were collected. Eighty-two F₂ plants were grown in the greenhouse as a CC genome mapping population during 2004. DNA extraction and SSR marker analysis: Total genomic DNA was isolated from the parental lines, F₁ and F₂ plants, using the BioRobot EZ1 and EZ1 DNA kits. 12 SSR markers were used for analysis of F₂ population. The PCR reactions were performed as described by Wu and Tanksley (1993). The polymorphism between W1519 and SL6 with respect to these markers was assessed following PCR conditions: a denature period of 2 min at 94ºC followed by 35 cycles of 15s at 94ºC, 30s at 55ºC, 1 min at 68ºC and 3 min at 68ºC for final extension. Reactions were carried out in a volume of 10 μl. The amplified primer PCR products were separated in 6% in polyacryalmide gel. The gels were stained with ethidium bromide (0.5 μg/ml) and photographed under UV light (Sambrook and Russel 2001) and the banding patterns were recorded.

Source of Information: Plant Tissue Cult. & Biotech. 16(1): 37-43, 2006 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The F₂ population showed transgressive segregation for days to heading. Ten plants flowered earlier and another 13 later than the two parents. Segregation for other characters. The results revealed various segregation patterns for the characters analyzed. Some characters had a normal distribution such as the internode colour and leaf size. Other characters showed segregation towards one of the parents like panicle type towards the Uganda parent and auricle color and leaf morphology towards the Sri Lankan parent. Similar segregation patternhas been reported in mapping population of rice, corn and barley. The trait internode colour and leaf size showed monogenic pattern of inheritance where purple colour was dominant over green colour of internode and broad leaf shape was dominant over narrow leaf shape. In both the cases non-significant χ² value ranged from 0.046 to 0.690 at 5 per cent level of probability. The results obtained will be incorporated with others to enable the marker linked to resistance or interesting characters or the CC genome of Oryza to be compared with the genome map of rice

Knowledge Management: Journal