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Multiple Shoot Formation in Eggplant (solanum Melongena L.)

R. H. Sarker
Department of Botany, University of Dhaka, Dhaka

Sabina Yesmin
Department of Botany, University of Dhaka, Dhaka

M. I. Hoque
Department of Botany, University of Dhaka, Dhaka

Abstract/ Executive Summary:

Among the in vitro derived different explants such as cotyledonary leaf, hypocotyl, shoot tip and root of two local varieties, namely Singhnath and Kazla (BARI Begun-4) of eggplant (Solanum melongena L.) cotyledonary leaf was found to be the best for multiple shoot regeneration. High frequency direct organogenesis of shoots was achieved from cotyledonary leaf in MS supplemented with 1.0 mg/l BAP and 1.0 mg/l Kn. Anatomical studies using freezing microtome supported the formation of shoots through organogenesis. Proliferation and elongation of such shoots were obtained in hormone free MS. Moreover, the regenerated shoots produced healthy roots when they were cultured on MS without hormonal supplements. Following the formation of roots the in vitro raised plantlets were successfully established in soil. Viable seeds were obtained from the in vitro raised mature plants.

Keywords: Regeneration, Multiple shoot, Eggplant

Location:

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Brinjal

Objectives:

The present attempt was made to develop a suitable regeneration protocol for eggplant cultivars grown in Bangladesh

Methodology:

Seeds of the two varieties of eggplant (Solanum melongena L.) namely, Kazla (BARI Begun-4) and Singhnath were collected from the Vegetable Seed Division of Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur and East West Seed (Bangladesh) Ltd., respectively.For induction of roots, regenerated shoots (2.5 - 4 cm long) were excised and transferred to MS and half strength MS medium with 3 % sucrose without hormonal supplements. Another set of rooting experiments was conducted using MS supplemented with different concentrations of IBA, IAA and NAA. Following the development of sufficient root system plantlets were transferred to small plastic pots containing sterilized soil. The in vitro raised plantlets were acclimatized and then transferred to the field. Histological examinations during shoot formation were carried out using a freezing microtome (Coldtome, Sakura, Japan). Microtome slide preparation and observation were made following the methods as described by Sarker and Awal (1999).

Source of Information: Plant Tissue Cult. & Biotech. 16(1): 53-61, 2006 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The in vitro regeneration protocol described here is easily, reproducible, requires minimum hormonal supplements and genotype independent. In here the efficiency of regeneration system is further demonstrated by obtaining viable seeds from the regenerated plants. Moreover, the regeneration of plantlets achieved without the intervention of callus and this clearly indicates the possibility of obtaining true-to-type plantlets. This present protocol can effectively be used for the development of desired plant types following future genetic transformation in eggplant varieties.

Knowledge Management: Journal