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In Vitro Regeneration in Three Varieties of White Jute (corchorus Capsularis L.)

R. H. Sarker
Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

G. M. Al-Amin
Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

M. I. Hoque
Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

Abstract/ Executive Summary:

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compared to other two varieties to produce multiple shoots. Cotyledonary nodal explants of all the three varieties were found to produce maximum number of multiple shoots on MS supplemented with 0.2 mg/l BAP and 1.0 mg/l IAA. Best root induction was observed at the base of the in vitro regenerated shoots on half the strength of MS supplemented with 0.3 mg/l IBA. The in vitro grown plantlets were successfully transplanted into soil. They grew up to maturity, flowered and fruited like the control plants.

Keywords: Regeneration, White jute, Corchorus capsularis

Location: Plant Breeding and Biotechnology Laboratory, DU, Dhaka

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Jute

Objectives:

To establish an efficient in vitro plant regeneration system from different explants of C. capsularis which may be applied to obtain jute transgenics of desired traits using Agrobacterium mediated or other powerful techniques such as particle gun bombardment.

Methodology:

Seeds of the three cultivated varieties, namely CVL-1, CVE-3 and D-154 of white jute (Corchorus capsularis L.) collected from Bangladesh Jute Research Institute, Sher-e-Bangla Nagar, Dhaka were used in the present investigation.Various explants used for in vitro regeneration were collected from in vitro raised seedlings. For this purpose seeds were washed with detergent under running tap water followed by rinsing in 70% alcohol for 30 seconds and with 0.1% HgCl₂ solution for 25 to 30 mins. The seeds were then washed five times with sterilized distilled water. The surface sterilized seeds were soaked overnight in sterile distilled water or inoculated into sterile water soaked filter papers and water-agar medium for supporting seed germination and seedling development. Seed germination was found to be better on water-agar medium than sterile water soaked filter paper. Cotyledon was found to enlarge in size during germination on sterile water soaked filter paper. Such enlarged cotyledons were also used as explants for regeneration. Various explants such as, petiole-attached cotyledons (PC), cotyledonary nodes, mature embryos with or without cotyledons were used for in vitro regeneration. PC and cotyledonary nodes were collected from five to six days old seedlings. Mature embryo and embryo with cotyledon explants were isolated from overnight soaked seeds.

Source of Information: Plant Tissue Cult. & Biotech. 17(1): 11-18, 2007 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Results of the present investigation showed that it is possible to regenerate large number of multiple shoots from the PC explants on MS with 0.2 ml/l BAP and 1.0 mg/l IAA from CVL-1 and D-154. On the other hand, 2.5 mg/l BAP with 0.5 mg/l NAA for CVE-3. Root induction in all the three varieties were also identical on half strength MS containing 0.3 mg/l IBA. The regeneration protocol developed here has been proved reproducible and more or less genotype independent. This protocol may be effectively used for the improvement of different jute cultivars through Agrobacterium-mediated or any other suitable methods of genetic transformation

Knowledge Management: Journal