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Morphological Features Growth and Development of Dendrobium Sp. Orchid as Influenced by Nutrient Spray

M. I. Kabir
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

M. G. Mortuza
Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh

M. O. Islam
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The experiment was conducted to see the effect of nutrient spray on morphophysiological feature and growth of three orchid varieties namely Dendrobium Red Bull, D. Kasim Gold and D White 5 N. Results revealed that the morphophysiological and growth attributes significantly varied among the cultivars. D Red Bull showed the highest plant height, leaf length, leaf area and stem diameter among the varieties. D. White 5 N was superior in leaf number and total leaf area and D. Kasim Gold was superior in leaf area index and leaf width to the other varieties. On the other hand, the trend of increasing in leaf length, leaf width, leaf area index, leaf number, leaf area and total leaf area was the highest for N:P:K as 10:25:30. Plant height and stem diameter was maximum for N:P:K as 15:20:20. In conclusion, low level of nitrogen and high level of phosphorus and potassium was suitable for leaf length, leaf width, leaf area index, leaf number, leaf area and total leaf area while high level of nitrogen and low level of potassium was suitable for plant height and stem diameter.

Keywords: Fertilizer, Production protocol, Spray solution standardization

Location: Dipta Orchid Ltd. Dulma, Enayetpur, Fulbaria, Mymensingh

Start Date: 00-12-2009

End Date: 00-06-2010

Program Area: Variety and Species

Commodity: Orchid

Objectives:

1. To standardize a suitable fertilizer formulation contained N, P, and K at appropriate concentration to be sprayed in Dendrobium sp. orchids for their optimal growth, development and commercial production.

Methodology:

The seedlings of Orchid plants (Dendrobium Red Bull, Dendrobium Kasim Gold and Dendrobium white 5 N) were collected from Dipta Orchid Ltd. Dulma, Enayetpur, Fulbaria, Mymensingh. The age of Clone was about six months. These clones were separated from mother plant and cultured for six months with coconut shell as supporting material. The experiment was laid out in Completely Randomized Block Design (RCBD) with 12 treatments and each treatment was replicated three times. The cultivars were treated with spray formulations prepared by mixing Urea, Tripple Super Phosphate (TSP) and Muriate of Potash (MP) maintaining N, P and K at different ratio. Formulation was prepared by following the thumb rule of BARI where total amount of fertilizer was 700 g in 172 liter water recommended for use as spray. The different ratios of N, P and K in spray nutrient formulation applied from December, 2009 to June, 2010. The formulation used in present research contained different ratio of N, P and K. The ratio was changed over growth stage of orchid. Spray formulation used in early stages contained higher N and gradually reduced in later stages where P and K were increased. Experimental data, such as, plant height (cm), leaf number, leaf length (cm), leaf wide (cm), leaf area (cm²), total leaf area (cm²), leaf area index (LAI) and stem diameter (cm) were recorded. To investigate the general anatomical structure of root, stem and leaf, a thin (about 10 μM) section was prepared from fresh plant material using sharp blade. The thin sections were stained in 1.0% safranin and mounted on slide. A cover sleep was set on thin section and observed under microscope with high resolution, progressive scan digital camera system (Model Olympus BX 41 and camera DP 20) and photos were taken. All collected data were analyzed by following the analysis of variance (ANOVA) technique and mean differences were adjudged by Duncan’s Multiple Range Test (DMRT) using a computer operated programme named MSTAT-C.

Source of Information: J. Environ. Sci. & Natural Resources, 5(1): 309-318, 2012; ISSN 1999-7361

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Results revealed that the morphophysiological and growth attributes significantly varied among the cultivars. D Red Bull showed the highest plant height, leaf length, leaf area and stem diameter among the varieties. D. White 5 N was superior in leaf number and total leaf area and D. Kasim Gold was superior in leaf area index and leaf width to the other varieties. On the other hand, the trend of increasing in leaf length, leaf width, leaf area index, leaf number, leaf area and total leaf area was the highest for N:P:K as 10:25:30. Plant height and stem diameter was maximum for N:P:K as 15:20:20.

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