Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Pathogenicity and Immunosuppressive Properties of Gm-97 Strain of Infectious Bursal Disease Virus in Commercial Broiler Chickens

Rozina Murmu
Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University,Dinajpur-5202, Bangladesh.

Md. Nazrul Islam
Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University,Dinajpur-5202, Bangladesh.

Most. Sogra Banu Juli
Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University,Dinajpur-5202, Bangladesh.

Md. Abu Sayed Khan
Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University,Dinajpur-5202, Bangladesh.

S. M. Harun-ur-Rashid
Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University,Dinajpur-5202, Bangladesh.

Ferdaus Mohd. Altaf Hossain
Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh.

Md. Masudur Rahman
Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh.

Abstract/ Executive Summary:

The current study was conducted to evaluate the pathogenicity and immunosuppressive effects of GM-97 strain of infectious bursal disease virus in commercial broiler chickens. A total of 500 broiler chickens were vaccinated with the virus through oral route at 10 and 17 days of age (102-103 EID50/dose). Chickens were also vaccinated with Newcastle disease virus (Hitchner B1) orally at 14 and 21 days old. Chickens were euthanized (at 12, 14, 16, 20, 23, 26 days of age) after measuring body weight. Bursa of Fabricius was examined for any gross lesion, weighed and processed for histological investigations. Bursa to body weight ratio and bursal lesion scoring were made to evaluate pathogenicity of the virus. Blood samples were analyzed for antibody response to ND vaccine virus using HI test. Results showed that the GM-97 strain of IBDV induced mild to moderate depletion of lymphoid cells in the center of bursal follicles and non-significant difference in bursa to body weight ratio amongst vaccinated and unvacci-nated chickens. Chickens responded well to ND vaccine by mounting high level of serum NDV specific HI antibody titers. It can be concluded from the present study that GM-97 strain of IBDV has mild pathogenicity but is not immunosuppressive.

Keywords: Commercial chicken, GM-97 strain, IBD vaccine, Immunosuppression, Pathogenicity

Location: Nourish Poultry and Hatchery Ltd., Bangladesh.

Start Date:

End Date:

Program Area: Pest Management

Commodity: Chicken

Objectives:

To evaluate the pathogenicity and immunosuppressive effects of GM-97 strain of infectious bursal disease virus in commercial broiler chickens.

Methodology:

Experimental chickens and their management: A total of 500 unvaccinated Cobb-500 day old chicks (DOC) were received from the Nourish Poultry and Hatchery Ltd., Bangladesh. The chicks were reared in a relative isolation under farming condition at Polash Poultry Farm, Saidpur under Nilphamari district of Bangladesh. The farm was thoroughly cleaned, disinfected with a household phenolic disinfectant (Phenyl) and fumigated before placing the DOC in the farm. Chicks were provided commercial feed and water ad libitum under relatively optimum temperature and humidity conditions and strict bio security measures were adopted.

Vaccine and vaccination schedule: A freeze-dried live vaccine (HIPRAGUMBORO®GM97, Hipra, Bangladesh Ltd.) containing the GM-97 strain of IBDV was used to vaccinate the birds. The chickens were immunized orally at 10 days of age through drinking water (102-103 EID50/dose) and boosted at 17 days old (7 days post-primary vaccination) using the same dose and route of administration as recommended by the manufacturer. Another freeze-dried live vaccine (Hitchner B1 strain ND Vaccine, Hipra, Bangladesh Ltd.) was used to immunize chickens against Newcastle disease (ND) at 14 and 21 days of age through drinking water (7.5 Log10EID50/dose). A group of chickens (n=10) were reared in the same farm in relative isolation without any vaccination and considered as negative control group.

Source of Information: J. Adv. Vet. Anim. Res., 1(1): 1-7, March 2014

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Based on our findings, the GM-97 strain of IBDV contained in HIPRAGUMBORO®GM97 showed mild pathogenicity in vaccinated chickens which did not have immunosuppressive effects. However, immuno-genicity and protective efficacy of the vaccine virus against currently circulation virulent field strain of IBDV (vvIBDV) need to be further addressed

Knowledge Management: Journal