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In Vitro Propagation of Abrus Precatorius L. - a Rare Medicinal Plant of Chittagong Hill Tracts

Animesh Biswas
Institute of Biological Sciences, Rajshahi University, Rajshahi

Mohashweta Roy
Institute of Biological Sciences, Rajshahi University, Rajshahi

M. A. Bari Miah
Institute of Biological Sciences, Rajshahi University, Rajshahi

S. K. Bhadra
Department of Botany, Chittagong University, Chittagong

Abstract/ Executive Summary:

An efficient protocol was developed for in vitro propagation of Abrus precatorius L. through induction of indirect organogenesis in nodal segment derived callus tissue. Yellowish-green nodular callus was induced at the cut surface of the nodal segments cultured on MS fortified with 5.0 mg/l BAP and 0.5 mg/l NAA. The callus differentiated into adventitious shoots when it was subcultured on to MS supplemented with 3.0 mg/l BAP + 0.5 mg/l Kn + 0.5 mg/l NAA. On an average 6.87 ± 0.26 shoots/culture developed. These microshoots were rooted in half-strength MS containing 1.0 mg/l IBA and the rooted plantlets were transferred to soil after proper acclimatization.

Keywords: Abrus precatorius, Medicinal plant, Callus culture, Propagation

Location:

Start Date:

End Date:

Program Area: Resource Development and Management

Commodity:

Objectives:

The present investigation reports the in vitro propagation technique that can be used as a potential tool for large scale production of this medicinal plant.

Methodology:

Mature seeds of Abrus precatorius L. were collected from a local herbalist of CHT and sown in earthen pots for raising seedlings. Juvenile twigs from one-year-old mature plants were used as source of explants. Juvenile twigs were surface sterilized with HgCl₂ solution (0.1% w/v) for four - six min. Thereafter, five washes were done with sterile distilled water. Nodal segments of twigs were cut (0.5 cm) and cultured on 8% (w/v) agar solidified MS supplemented with various growth regulators (NAA, IAA, IBA, BAP and Kn) at different concentrations and combinations. Subculturing was done at an interval of 14 - 20 days. Once the shoot buds developed, they were further cultured for elongation in the same medium. Elongated shoot buds were rooted on half strength MS fortified with different concentrations of auxins (NAA and IBA) alone. The pH of the medium was adjusted to 5.8 before autoclaving. All cultures were incubated at 25 ± 2ºC under 16/8 hr photoperiod. After 12 weeks, plantlets with roots were successfully planted in pot soil through gradual acclimation

Source of Information: Plant Tissue Cult. & Biotech. 17(1): 59-64, 2007 (June)

Article Link (Online Journal):

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Total Budget (Tk.) :

Achievement/Findings:

Rooting experiments were conducted in MS supplemented with 0.1-1.0 mg/l either NAA or IBA. Medium containing 1.0 mg/l IBA proved to be the most effective for rooting of microshoots than that of any concentration of NAA. In this medium the highest per cent (70) and number (3.23 ± 0.27) of root formed at the cut end of microshoots within two weeks of culture. The effectiveness of IBA in rooting has been reported in many medicinal plants, Shoots with strong and stout root system were acclimatized outside growth chamber for one week and then transferred to earthen pots placed in natural environment containing mixture of soil and manure (1 : 1). Seventy five per cent plants survived in nature.

Knowledge Management: Journal