Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Hormonal Effects on in Vitro Regeneration of Ricinus Communis L. Cultivar Shabje

M A Rahman
Biotechnology Laboratory, Institute of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh

M A Bari
Biotechnology Laboratory, Institute of Biological Science, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

Context: Castor is the best candidate plant to the venture of green energy sector. In Bangladesh castor is widely grown and we can reap the benefit from castor by the application of different biotechnological approaches. Genetic engineering appears as one of the necessary tools for the improvement of cultivars of castor. Therefore, an efficient regeneration protocol for castor needs to be established, which is rapid, reproducible and applicable to a broad range of genotypes. Objectives: To establish a high frequency plant regeneration system from the seedling explants of Ricinus communis L. cultivar shabje using tissue culture technique. Materials and Methods: The proliferating axillary shoots were established on modified MS medium supplemented with different concentrations of BA, Kn and 2ip using explants from the in vitro grown seedlings of R. communis. Results: Explants from the seedlings raised on cytokinin enriched medium showed early sprouting and development of axillary shoots than those taken from the seedlings grown on cytokinin omitted medium. Shoot proliferation efficiency of cotyledonary node explant was noted two times greater than that of shoot tip explant. MMS1 medium fortified with 1.5-2.5 mg/l BA showed the best performance and yield 5-10 axillary shoots within 6 weeks of culture. Microcuttings prepared from in vitro proliferated shoots were rooted on MMS2 medium. The best rooting (92.7%) obtained on ½MS fortified with 0.2 mg/l of IBA accompanied by 0.6 mg/l of AgNO₃. Conclusion: Best media formulation for shoot proliferation in Ricinus cultivar was MS medium fortified with 2.0 mg/l BA and its recalcitrant property could be minimized in some extent by using of AgNO₃ and charcoal in case of root induction.

Keywords: In vitro regeneration, Seedling explants, Ricinus communis.

Location: Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Castor

Objectives:

To establish a high frequency plant regeneration system from the seedling explants of Ricinus communis L. cultivar shabje using tissue culture technique.

Methodology:

The explants used in this investigation were collected from 4 week old seedlings raised in vitro on MS medium. Seeds were collected from the research field of Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh. Seeds were washed thoroughly under running tap water and then treated with 1% savlon supplied by ACI and 2-3 drop of Tween-80 for about 10 min. This was followed by successive three washing with distilled water to make free the seeds from savlon and Tween-80, surface sterilization was carried out with 0.1% HgCl₂ for 6-7 min followed by gentle shaking. After this treatment, the seeds were rinsed 4-5 times in sterile distilled water to make free the seeds from HgCl₂. Sterilized seeds were partially decoated and aseptically germinated in glass bottle containing 50 ml semisolid MS (Murashige and Skoog 1962) medium either with or without 1 mg/l BA. The aseptic seedlings attained a height of 8-10 cm within 3-4 weeks under incubation. Two types of explants (1 cm shoot segments) consisting of cotyledonary node and shoot tips were prepared from in vitro raised seedlings under completely sterile condition and cultured on different nutrient media containing cytokinin for proliferating axillary shoots. Following sequential subculturing and multiplication of primary culture, a stock of axillary shoot cultures was raised. For in vitro rooting of shoots, 1-3 cm long microcuttings were prepared from in vitro proliferated shoots. Rooting was induced by culturing microcuttings on media containing different concentrations of auxins. For axillary shoot proliferation MMS1 (MS with half strength of macronutrients and full strength of micronutrients and vitamins) media supplemented with three types of cytokinins were used. For root formation in the in vitro regenerated shoots, only MMS₂ (MS with half strengths of both macro and micronutrients and full strength of vitamins) with different concentrations and combinations of auxins and additives were used. The media were supplemented with 20 g/l of sucrose and gelled with 8 g/l of agar (BDH). The pH of the medium was adjusted to 5.7-5.8 prior to autoclaving at 121ºC under 1.1 kg/cm² pressure for 20 minutes. Unless otherwise mentioned, all cultures were maintained in growth room under 16 h photoperiod with a light intensity of 2000-3000 Lux (50-70 mE. m^-2. S^-1).

Source of Information: J. bio-sci. 18: 121-127, 2010 ISSN 1023-8654

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Best media formulation for shoot proliferation in R. communis cultivar shabje was MS medium fortified with 2.0 mg/l BA and its recalcitrant property could be minimized in some extent by using of AgNO₃ and charcoal in case of root induction.

Knowledge Management: Journal