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Performance of Phosphate Solubilizing Bacteria with Various Phosphorus Levels on Wheat in Pot Culture

M. A. Haque
Soil Microbiology Lab., Soil Science Division, BINA

M. A. Sattar
Director General Office, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

M. R. Islam
Department of Soil Science, BAU, Mymensingh

M. A. Hashem
Department of Soil Science, BAU, Mymensingh

M. K. Khan
Soil Microbiology Lab., Soil Science Division, BINA

Abstract/ Executive Summary:

A pot experiment was conducted to investigate the effect of different phosphate solubilizing bacteria (PSB) with various levels of inorganic phosphorus (P) on the growth and yield of wheat with three soil at the Microbiology laboratory, Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Four levels of P fertilizer (P₀ : Without P, P₂₄ : 24 mg P kg^-1 soil, P₃₆ : 36 mg P kg^-1 soil and P₄₈ : 48 mg P kg^-1 soil) were assigned as main factors and six PSB inoculants with an uninoculant (I₀, MR1, IL1, IW1, IC2, RC1and MW1) were assigned as sub factors in a factorial CRD. The soil used for the experiment belong to three Agroecological Zones (AEZs) such as AEZ 9 from the BAU farm, Mymensingh, AEZ 28 from the BADC farm, Madhupur, Tangail and AEZ 11 from the BINA substation farm, Ishurdi, Pabna. Eight kilogram soils were taken in each of the plastic pot. PSB were applied as broth inoculants with the wheat seeds (var. Shatabdi) before sowing. Irrespective of the PSB inoculants, 36 mg P kg^-1 soil gave the highest grain and straw yield of wheat pot^-1 with Mymensingh and Mdhupur soil while 48 mg P kg^-1 soil showed the highest wheat yield pot^-1 with Ishurdi soil. Among the PSB inoculants, MR1 showed the maximum grain and straw yields of wheat pot-1 followed by MW1. The PSB inoculant MR1 with 36 mg P kg^-1 soil had better performance in terms of total P uptake and grain and straw yields of wheat.

Keywords: Phosphate solubilizing bacteria, P uptake, Wheat yield

Location: Microbiology laboratory, Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

Start Date: 00-11-2011

End Date: 00-03-2012

Program Area: Crop-Soil-Water Management

Commodity: Wheat

Objectives:

1. To evaluate the effects of different PSB inoculants on wheat in pot culture with diverse soils.

Methodology:

The soils used for the pot experiment belongs to three Agroecological Zones (AEZs) namely: Old Brahmaputra Floodplain (AEZ 9) from the Bangladesh Agricultural University Farm (BAU farm), Mymensingh, Madhupur Tract (AEZ 28) from the Bangladesh Agriculture Development Corporation Seed Multiplication Farm (BADC farm), Madhupur, Tangail and High Ganges River Floodplain Soil (AEZ 11) from the BINA substation farm, Ishurdi, Pabna. The soils were collected at a depth of 0-15 cm from each site by means of spade. Eight kilogram processed soils were taken in each of the plastic pot (23 cm × 21 m). The experiment was conducted in a factorial CRD. Four levels of P fertilizer (P₀ : Without P, P₂₄ : 24 mg P kg^-1 soil, P₃₆ : 36 mg P kg^-1 soil and P₄₈ : 48 mg P kg^-1 soil) were assigned as main factors and six PSB inoculants with an uninoculant control (I₀ : Uninoculated control, I₁: PSB inoculants MR1, I₂: PSB inoculant IL1, I₃: PSB inoculant IW1, I₄: PSB inoculant IC2, I₅: PSB inoculant RC1 and I₆: PSB inoculant MW1) were assigned as sub factor treatments for each soil. Each treatment was replicated thrice. P fertilizer was applied as per treatment plan from triple super phosphate (TSP). Potassium was applied @160 mg K kg^-1 soil from muriate of potash (MoP) as basal in each pot. Nitrogen fertilizer @ 240 mg N kg^-1 soil was applied as urea in three equal splits in all the pots. Wheat seeds (var. Shatabdi) were soaked (except uninoculated control pots) separately with the culture broth of each PSB inoculant containing 1012 cells mL^-1 broth for 30 min before sowing. Inoculated and uninoculated wheat seeds were sown separately into the pots. After germination of wheat seeds, only five plants were kept for normal growth in each pot. The wheat plants were harvested when attained at full maturity and yields were recorded in g pot^-1. The initial soils were analyzed for texture, organic carbon content, cation exchange capacity, total nitrogen, available phosphorus, total phosphorus, exchangeable potassium following standard methods. Grain and straw samples were digested following standard method and phosphorus content in the digested samples was determined colorimetrically by vanado-molybdo yellow colour method. After determination of P content in grain and straw samples of wheat, P uptakes were calculated using the value of P contents. Analysis of variance was performed in factorial CRD followed by F-test to examine whether the treatment effects were significant using MSTATC computer pakage program.

Source of Information: J. Environ. Sci. & Natural Resources, 6(1): 221 - 226, 2013; ISSN 1999-7361

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that among the PSB inoculants either MR1 or MW1 with reduced rate of P fertilizer showed better performance in wheat yield than P fertilizer alone.

Knowledge Management: Journal