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The Study of Fillet Proximate Composition, Growth Performance and Survival Rate of Striped Catfish (pangasius Hypophthalmus) Fed with Diets Containing Different Amounts of Alpha-tocopherol (vitamin-e)

Syeda Mushahida-Al-Noor
Department of Fisheries, Faculty of Agriculture, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh

Md Delwer Hossain
Department of Fisheries, Faculty of Agriculture, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh

Md Amirul Islam
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

Context: Alpha-tocopherol has significant effect on the growth performance and fillet proximate composition of Striped Catfish (Pangasius hypophthalmus). Objectives: To study the effect of DL-alpha tocopheryl acetate on the fillet proximate composition (moisture, crude protein, crude lipid, ash and alpha-tocopherol), survival rate and growth performance (SGR, weight gain, PWG) of P. hypophthalmus. Materials and methods: Striped Catfish were fed with three trial diets (diet-1, diet-2 and diet-3) were prepared by adding different amounts of alpha-tocopheryl acetate (ATA, 0, 90 and 300 mgKg-1 diet), over a period of 122 days. In each pond, 10% of the total fish were randomly selected and individually weighed once every fortnight and growth parameters were determined after each weighing. Moisture, dry matter, crude protein, lipid, ash and alpha-tocopherol of the experimental diets and fish fillet were determined by Conventional procedure, standard test method, micro-kjeldahl method, Bligh and Dyer method, standard methods and chromatographic method, respectively. Results: Fish that were fed 90 mg kg-1 and 300 mg kg-1 ATA supplemented diets had significantly higher growth performance than fish that were fed only farm processed diet (P<0.05). The fillet protein, lipid content and alpha tocopherol level of the fish flesh were increased due to ATA supplementation in diets. The vitamin-E levels in fish fillet reflected dietary ATA levels. These results showed that fish on Diets 2 (supplemented with 90 mgKg-1 ATA) and Diet 3(supplemented with 300 mg kg-1 ATA) had significantly better production and resulted better flesh quality of fish than those on Diet 1 (without ATA supplementation). Conclusion: Scanty information is available on the fillet proximate composition and growth performance of P. hypophthalmus fed with diets containing different amounts of alpha-tocopherol. The present study provides baseline information for establishing improved and profitable cultured system of the fish in impounds water of Bangladesh.

Keywords: Nutrition, Vitamin supplementation, Alpha-tocopheryl-acetate, Weight gain, Flesh’s proximate composition.

Location: University of Rajshahi’s Faculty of Agriculture, Department of Fisheries, Bangladesh

Start Date: 00-07-2011

End Date: 00-11-2011

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

To study the effect of DL-alpha tocopheryl acetate on the fillet proximate composition (moisture, crude protein, crude lipid, ash and alpha-tocopherol), survival rate and growth performance (SGR, weight gain, PWG) of P. hypophthalmus.

Methodology:

Fish and Experimental Diets: The study was conducted at University of Rajshahi’s Faculty of Agriculture, Department of Fisheries, Bangladesh (July to November, 2011). The experimental diets were prepared by adding 0 mg kg-1 (Diet 1), 90 mg kg-1 (Diet 2) and 300 mg kg-1 (Diet 3) of DL-alpha-tocopheryl acetate dry powder (ACI Company Ltd., Dhaka, Bangladesh) to a farm-made striped catfish diet produced with rice bran, boiled rice and mustard oil cake. The actual levels of vitamin-E in the experimental diets were 90.2 ± 0.2, 192.5 ± 0.4 and 386.9 ± 0.3 mg kg-1 (because the feed ingredients also contains indigenous vitamin E), respectively, as shown in Table 1. Striped catfish fingerlings with a mean initial weight of 125.05 ± 0.50 g (±SE, n = 200) were randomly allocated to three treatments (T1, T2, T3 with two replication each) in six ponds and fed the experimental diets once daily for 122 days. The daily feeding rations were adjusted to 5% of body weight. Growth Performance: In each pond, 10% of the total fish were randomly selected and individually weighted once every two weeks, and daily rations were determined after each weighting. The body weight gain (BWG) was calculated using the following equation: BWG (g) =final weight (g)-initial weight (g). The specific growth rate (SGR) was calculated using the following equation: SGR (%bwd-1) = [(ln final weight–ln initial weight) x 100 / time in days]. The percentage weight gain (PWG) was calculated using the following equation: PWG (%) = [(final weight– initial weight) x 100 / initial weight]. The survival rate was calculated using the following equation: Survival rate (%) = [(Number of harvested fish/Number of stocked fish) x 100]. Proximate Analysis: Twenty random samples, each month were killed and submitted for analysis of fillet composition. The fillets were blended prior to proximate analysis. Moisture, dry matter, crude protein, lipid, ash and alpha-tocopherol of the experimental diets and fish fillet were determined by Conventional procedure (ICOMR, 1971), Standard test method (ASTM 2003), Micro-kjeldahl method (Rangama 1979), Bligh and Dyer method (1959), Standard methods (AOAC 1998) and Chromatographic method (Huo et al.1999). Statistical Analysis: The difference between the growth performance and body composition among groups were analyzed with one-way analysis of variance (ANOVA) and Duncan’s multiple range test (Duncan 1955) with a statistical package program (SSPS version 11.0) for P<0.05 at the end of feeding trials.

Source of Information: J. bio-sci. 20: 67-74, 2012 ISSN 1023-8654

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, the conducted study demonstrated that diets containing different amount of alpha-tocopheryl acetate affect growth performance and led to increased vitamin E and lipid content in the fish flesh. These results suggest that feeding the fish diets containing 90 mgKg-1 and 300 mgKg-1 alpha-tocopheryl acetate results in higher flesh quality and comparatively batter growth in case of P. hypophthalmus compared to diet- 1(diet without alpha-tocopheryl acetate).

Knowledge Management: Journal