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Role of Oyster Mushroom (pleurotus Ostreatus) Against Cecal Coccidiosis in Cobb 500 Broiler Chicken

M. Z. Hossain
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. Akter
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. A. H. N. A. Khan
Deapartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. M. Ashraf
Deapartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. K. Islam
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

A total of 25 day old Cobb 500 broiler chicks were purchased and randomly divided into five groups (A, B, C, D and E). Group A served as noninfected, non-supplemented control. Group B were infected and non-supplemented while chicks of group C, D and E were supplemented oyster mushroom (Pleurotus ostreatus) @ 50, 100 and 150 mg/kg body weight respectively with feed from 2-28 day of age. The chicks of group B, C, D and E were challenged orally with 1 x 104 sporulated oocysts of Eimeria tenella on 10th day. The anticoccidial efficacy of oyster mushroom was evaluated based on oocysts counts per gram (OPG) of feces, weight gain, morbidity and mortality, necropsy findings and histopathology. Concerning the mean OPG count, body weight gain (p<0.01), morbidity and mortality the highest performance (p<0.01) was detected in group D and E on day 28 among the supplemented groups whereas group B showed the lowest performance. Group D and E showed the best results in regards of body weight, OPG counts, morbidity, mortality and post mortem findings as compared to healthy control (group A). So it could be concluded that supplementation of 100-150 mg oyster mushroom/kg body weight reduces the development of cecal coccidiosis in chicken.

Keywords: Oyster mushroom; Eimeria tenella; OPG; Body weight; Morbidity; Mortality.

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Chicken, Mushroom

Objectives:

The present study was conducted to evaluate the role of oyster mushroom supplementation at different doses against cecal coccidiosis in Cobb 500 broiler.

Methodology:

Experimental protocol and diets: To conduct the experiment, a number of 25 day old of Cobb 500 broiler chicks were collected from local market and equally divided into five groups (group A, B, C, D and E), each of which containing 5 chicks. Chicks of group A were maintained as noninfected, non-supplemented control. Chicks of group B were infected and non-supplemented whereas chicks of group C, D and E served as supplemented groups. Dried oyster mushroom (Pleurotus ostreatus) was collected from Horticulture Center, BAU First Gate, Mymensingh. The Dried mushroom was grinded to turn it into powder form. From day 2 up to day 28 of age, chicks of group C, D and E were supplemented with oyster mushroom powder at a dose rate of 50, 100 and 150 mg/kg body weight respectively with feed. Body weight of chickens was recorded on weekly basis. Oocyst per gram (OPG) of feces was counted following McMaster technique on day 28 of age of the chickens. Post-mortem examinations of the broilers were performed at the end of experiment (day 28). Ceca, intestine, liver and spleen were examination. The gross post-mortem findings of these organs were recorded.

Histology: The ceca was excised from each bird, immersed in chilled phosphate-buffered saline and blotted dry. A 4 mm section of the ceca was placed into a histological cassette. The cassette containing the cecal section of each bird was individually immersed in a 10% (v/v) buffered formalin phosphate solution for fixing and subsequent staining. The formalin-fixed, paraffin-embedded tissue samples were ultrasectioned (4–5 μm thickness), stained with hematoxylin and eosin and examined under a light microscope (Olympus).

Statistical analysis: All data were expressed as mean ± SE, group mean values of weight gain and oocysts output of the chicks were compared by 't' test with N, + N2-2 degrees of freedom. Statistical significance was set at p < 0.05. Statistical analysis was performed using SPSS software version 17 (SPSS Inc., Chicago, IL, USA).

Source of Information: J. Sci. Res. 5 (1), 185-193 (2013)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the present research it could be concluded that 100-150 mg oyster mushroom powder supplementation/kg body weight would be the best prophylactic solution against cecal coccidiosis than other doses. This study also suggests that oyster mushroom powder supplementation can prevent low growth rate of broilers due to coccidial oocyst ingestion in chickens. There is not any significant adverse effect in chicken due to this type of supplementation as no remarkable lesion was recorded in liver, spleen, bursa and thymus. However, in this study only one species of Eimeria (E. tenella) oocysts was used. So, further study is needed to determine the prophylactic efficacy of oyster mushroom against other species of Eimeria and these researches would provide a clear idea on the efficacy of oyster mushroom against chicken coccidiosis.

Knowledge Management: Journal