Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Plant Regeneration in Brassica Spp.

Sanjida Rahman Mollika
Plant Breeding & Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

R. H. Sarker
Plant Breeding & Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

M. I. Hoque
Plant Breeding & Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka

Abstract/ Executive Summary:

MS with different concentrations and combinations of hormones were used for in vitro multiple shoot regeneration in two varieties of Brassica juncea, namely, BARI Sarisha‐11 and BARI Sarisha‐16 and one variety of Brassica campestris, Tori‐7. The highest percentage of responsive explants towards the regeneration of shoots was obtained on MS with 2.0 mg/l BAP, 0.2 mg/l NAA and 0.5 mg/l Kn in case of BARI Sarisha‐11 and BARI Sarisha‐16. For Tori‐7, 3.0 mg/l BAP and 0.2 mg/l NAA was best for obtaining maximum number of shoots per explant. Among three varieties, BARI Sarisha‐11 showed best response in terms of shoot regeneration as well as number of shoot per explant. Days required for induction of shoots was also recorded to be lowest in BARI Sarisha‐11. Best root induction in BARI Sarisha‐11 and BARI Sarisha‐16 was achieved on hormone free MS. After proper hardening the in vitro regenerated plantlets were successfully transplanted into soil. Interestingly some of the in vitro regenerated shoots produced in vitro flowers on regeneration media as well as hormone free MS.

Keywords: Plant regeneration, Brassica juncea, Brassica campestris

Location:

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

Considering the importance of Brassica spp. in the present investigation attempts were made to establish a genotype independent regeneration protocol for locally available oilseed Brassica spp.

Methodology:

Three varieties of Brassica spp., namely Brassica juncea var. BARI Sarisha‐11 and BARI Sarisha‐16 and Brassica campestris var. Tori‐7 were collected from the Oil Seed Division of Bangladesh Agricultural Research Institute (BARI) and used in the present investigation. The surface sterilized seeds were inoculated on to half strength of MS with 2% sucrose and 0.8% agar for germination and seedling development. The cultured seeds were kept in dark condition till the germination and then transferred to 16 hrs light condition at 25 ± 2ºC in growth room. Normally germination took place within 2 ‐ 3 days of seed inoculation. Explants like cotyledonary leaf with or without petiole, petiole without cotyledon, cotyledonary node, hypocotyl, epicotyl and leaf segment were excised from germinating seeds. Four to five days old seedlings were used as the source of explant. Isolated explants were cultured on MS containing BAP, NAA and Kn singly or in combinations for regeneration. In vitro regenerated shoots were subcultured regularly to fresh medium at an interval of 12 ‐ 15 days for further multiplication. Elongated shoots were separated and cultured on rooting medium for root formation. About 2 ‐ 3 cm long shoots were separated and cultured on rooting medium containing full and half strengths of MS without hormonal supplement or with different concentrations and combinations of IBA and NAA. The plantlets with sufficient root system were then transplanted to small pots containing sterilized soil. Pots were covered with transparent perforated polythene bags. After proper hardening, plantlets were transferred to natural environment.

Source of Information: Plant Tissue Cult. & Biotech. 21(2): 127-134, 2011 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Interestingly it was recorded that the in vitro regenerated shoots of BARI Sarisha‐11, BARI Sarisha‐16 and Tori‐7 produced in vitro flowers on regeneration media. These in vitro flowers were relatively smaller than these of in vivo produced flowers. This result revealed that regenerated shoots may synthesize flower inducing hormone in their body and induced flowering spontaneously without any external flowering hormonal supplement. After sufficient development of roots, plantlets of varieties BARI Sarisha‐11 and BARI Sarisha‐16 were successfully transplanted into small plastic pots. Using this method the survival rate of the transplanted plantlets was 100%. The plantlets survived in the pots containing soil. The plantlets of BARI Sarisha‐11 and BARI Sarisha‐16 flowered within 2.5 months and they flowered throughout the season. Seeds were harvested from the mature plants. Plants of BARI Sarisha‐11 produced by culture also established in the field. The harvested pods developed from the in vitro raised plant BARI Sarisha‐11. Based on the results it may be concluded that regeneration protocol developed in the present investigation for BARI Sarisha‐11 and BARI Sarisha‐16 is reliable and be effectively utilized for genetic transformation of Brassica species.

Knowledge Management: Journal