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Production of Oyster Mushroom on Different Substrates Using Cylindrical Block System

R. C. Dey
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

K. M. Nasiruddin
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. S. Haque
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. A. Z. Al Munsur
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The experiments were conducted at the Mushroom and Tissue Culture Laboratory, Horticulture Demonstration and Training Center (HDTC), DAE, Keyotkhali, Mymensingh during the period from February to May 2006. Oyster mushroom was cultivated on different substrates viz. paddy straw, sugarcane bagasse and mustard straw using cylindrical block system to find out suitable substrate. Different substrates significantly affected the number of primordia and fruiting bodies, and the amount of fresh weight or yield of Oyster mushroom in cylindrical block system. The highest number of primordia and fruiting bodies, and the amount of fresh weight was obtained with sugarcane bagasse in all flushes whereas, the lowest with mustard straw.

Keywords: Cylindrical block system, Oyster mushroom, Production, Substrates

Location: Mushroom and Tissue Culture Laboratory, Horticulture Demonstration and Training Center (HDTC), DAE, Keyotkhali, Mymensingh

Start Date: 00-02-2006

End Date: 00-05-2006

Program Area: Resource Development and Management

Commodity: Mushroom

Objectives:

To indetify the production of oyster mushroom on different substrates using cylindrical block system.

Methodology:

The methodology that was used in these experiments was standardized following the methods. For the preparation of substrate for spawn production wheat were washed, dried and boiled separately for 50 minutes and again dried in air by spreading on polythene sheet to remove surface moisture. After that 2 % CaCO₃ and 1 % chalk powder were mixed thoroughly on the polythene sheet. Then 250 g substrate were taken in to each polypropylene bag and plugged with cotton and rubber band. For the preparation of paddy, mustard straw and sugarcane bagasse chopped (2.54 cm size) and dry these substrates was soaked in water for about 24 hours to get wet and achieved 65-70 % of moisture content The next day, all these wet substrates were separated from water and excess water was removed properly. Then all these substrates were chopped and boiled for 1 hour. After cooling, the pasteurized substrates were kept on the concrete floor and limed with 10 % CaCO₃ solution. Then rice bran, gypsum and molasses were mixed with the pasteurized paddy mustard straw and rice bran & gypsum with the sugarcane bagasse. The substrates were thus ready to be used in mushroom cultivation. Three big polypropylene bags full of each type of substrates were placed in cylindrical block device. At first, a layer of prepared substrate was taken into a big polypropylene bag and then about 50 g of previously prepared spawn was spreaded on the outer side of substrate with cautions. This spawning process was repeated several times in the same manner. But last layer of spawn was covered with less amount of substrate. Then the bags were packed tightly and kept on cylindrical block device. All instruments, glass wares and culture media were sterilized by autoclaving with 15 PSI at 121^oC for 1-2 hours. The culture room of the laboratory was cleaned by gently washing with detergent followed by 70 % ethyl alcohol regularly. Data were recorded periodically during the growing season namely- 1^st flush, 2^nd flush and 3^rd flush. The observations were recorded on the number of primordia, fruiting bodies and fresh weight of mushroom. The data were statistically analyzed following the Randomized Complete Block Design (RCBD) and means were compared following Duncan’s Multiple Range Test (DMRT) test at 5% level of probability for interpretation of results.

Source of Information: Progress. Agric. 19(1) : 7-12, 2008 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the first flush, the number of primordia was found with significant variation in all substrates. The highest number of primordia (222.3) was found in sugarcane bagase and the lowest (170.3) in mustard straw. The similar trends were also observed in the 2^nd and 3^rd flush. The highest number of fruiting bodies (234.7) of Oyster mushroom was found in sugarcane bagasse and the lowest (179.7) in mustard straw in the 1^st flush. The number of fruiting bodies was found to vary significantly among all substrates. The similar trends were also observed in the 2^nd and 3^rd flush. The highest (1029.0 g) fresh weight of fruiting bodies was obtained in sugarcane bagasse, whereas the lowest (943.7 g) in mustard straw. These results were found statistically different in different substrates. The similar trends were also observed in the 2nd and 3rd flush. From the above study we found that, sugarcane bagasse performed best followed by rice straw and mustard straw for the production of Oyster mushroom using cylindrical block system.

Knowledge Management: Journal