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Evaluation of Antibody Production Against Newcastle Disease Virus After Immunization with Different Vaccines in Fayoumi Chicks

Shama Ranjan Barua
Advance Animal Health Co Ltd, Maijdy, Noakhali 3800, Bangladesh.

M Mansurul Amin
Department of Microbiology & Hygiene, Bangladesh Agricultural University(BAU), Mymensingh 2202, Bangladesh,

Shaiful Islam
Bangladesh Livestock Research Institute (BLRI), Regional Station, Baghabari, Sirajgonj 6770, Bangladesh.

Sukanta Chowdhury
Bangladesh Extension Education Services (BEES), Kazipara, Brahmanbaria 3400, Bangladesh, 5Barishal Government Veterinary College, Babugonj, Barishal 8210, Bangladesh.

M Shafiqul Islam Khan
Barishal Government Veterinary College, Babugonj, Barishal 8210, Bangladesh.

M Ali Asgar
Barishal Government Veterinary College, Babugonj, Barishal 8210, Bangladesh.

Abstract/ Executive Summary:

The research work was performed in the experimental sheds and virology laboratory of the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh during the period from January to November 2006 for the detection of persistence of maternallyderived antibody (MDA) against Newcastle disease virus (NDV) in Fayoumi chicks as well as evaluation of antibody production in chicks after vaccination. Five experimental groups of 110-day-old Fayoumi chicks were vaccinated for comparative study. Group A was vaccinated with baby chick ranikhet disease vaccine (BCRDV, F-strain), group B with Izovac B1 Hitchner® (B1 strain), group C with Cevac New L® (LaSota strain) and group D with Avinew® (VG/GA strain) through intraocular (i/o) route while group E was kept as unvaccinated control. Serum samples obtained from 10 randomly selected birds from each group on the occasion of 7, 13, 15, 17, 19 and 21 days post-vaccination (DPV) were subjected to haemagglutination inhibition (HI) test. It was found that persistence of MDA against Newcastle disease virus (NDV) retained until the age of day 20 in Fayoumi chicks and among the four commercial vaccines, Avinew® (VG/GA strain) appeared to be slightly superior compared to those of BCRDV (F-strain), Izovac B1 Hitchner® (B1 strain) and Cevac New L® (LaSota strain) in respect of HI antibody response. The F-values of HI titre among four vaccines were significantly different (p <0.01).

Keywords: Newcastle disease virus (NDV), Fayoumi chicks, Maternally-derived antibody (MDA), Haemagglutination inhibition (HI), Newcastle disease (ND) vaccines

Location: BAU,Mymensingh

Start Date: 00-01-2006

End Date: 00-11-2006

Program Area: Pest Management

Commodity: Chicken

Objectives:

1.To record the persistence of maternally-derived antibody (MDA) against NDV in Fayoumi chicks

2.To compare the antibody production level to BCRDV (F strains), Izovac B1 Hitchner® (B1 strain), Cevac New L® (LaSota strain) and Avinew (VG/GA strain) in Fayoumi chicks

Methodology:

Experimental chicks: A total 110 apparently healthy day-old-chicks of Fayoumi breed were collected from the Government Poultry Farm, Mirpur, Dhaka and carried to the experimental houses of the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh. The birds were supplied with feed (Quality Feed Co Ltd) and water adlibitum maintaining strict biosecurity. Newcastle disease vaccine (NDV) Lyophilized ND vaccines baby chick ranikhet disease vaccine (BCRDV, F-strain, lentogenic), Izovac B1 Hitchner®, (B1 strain, lentogenic), Cevac New L® (LaSota strain, lentogenic) and Avinew® (VG/GA strain, lentogenic) were collected from local market and were used during the experiment. The vaccines were stored and diluted during use according to the instruction of manufacturers. Experimental design: One hundred and ten-day-old Fayoumi chicks were grouped into five groups; viz., A, B, C, D and E, where group A, B, C and D contained 20 birds each and group E contained 30 birds. Prior to grouping and vaccination blood samples were collected to measure the maternally-derived antibody (MDA) on day 3 and day 7 of age of chicks, which were then divided into five groups (n = 5) as required. The birds of group A, B, C and D were vaccinated with BCRDV (F-strain, lentogenic), Izovac Hitchner B1 ® (B1 strain, lentogenic), Cevac New L® (LaSota strain, lentogenic) and Avinew®, (VG/GA strain, lentogenic) vaccines respectively through intraocular (i/o) route while birds of group E was kept as unvaccinated control. The vaccines were administrated at 7 days of age according to manufacturer’s direction. Blood samples were collected for sera from 10 randomly selected birds from 4 former group at 7, 13, 15, 17, 19 and 21 days post-vaccination (DPV) and from group E at the age of day 7, 10, 14, 16, 18, 20 and 22 for the determination of haemagglutination inhibition (HI) antibody titre using HI test.Newcastle disease vaccine virus: Laboratory cultures of B1 vaccine virus of Izovac B1 Hitchner® was propagated in fertile eggs (embryo of 10-day-old) of Fayoumi chicken and the allantoic fluid was used as antigen for haemagglutination (HA) and haemagglutination inhibition (HI) test. The presence of NDV in allantoic fluid was tested by slide haemagglutination (HA) test¹⁰

Source of Information: Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 31-35

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Serum samples obtained from 10 randomly selected birds from each group on the occasion of 7, 13, 15, 17, 19 and 21 days post-vaccination (DPV) were subjected to haemagglutination inhibition (HI) test. It was found that persistence of MDA against Newcastle disease virus (NDV) retained until the age of day 20 in Fayoumi chicks and among the four commercial vaccines, Avinew® (VG/GA strain) appeared to be slightly superior compared to those of BCRDV (F-strain), Izovac B1 Hitchner® (B1 strain) and Cevac New L® (LaSota strain) in respect of HI antibody response. The F-values of HI titre among four vaccines were significantly different (p <0.01).

Knowledge Management: Journal