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Characterization of Some Rhizobium Isolates and Their Effectiveness on Pea

M Solaiman Talukder
Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur 1706, Bangladesh,

ARM Solaiman
Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur 1706, Bangladesh,

ARM Solaiman
Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur 1706, Bangladesh.

Delowara Khanam
Division of Soil Science, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur 1701, Bangladesh.

Abstract/ Executive Summary:

Six isolates of Rhizobium were isolated from pea (Pisum sativum) to characterize their cultural properties and observe their effectiveness on host legume. In a laboratory study Rhizobium isolates showed characteristic pattern of reactions in respect of growth rate, colony characteristics and acid/alkali production on different growth media. The effect of inoculations of Rhizobium isolates on nodulation, growth and nitrogen fixation of pea were assessed by a pot experiment on a clay loam soil of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur. Inoculation treatments comprised of Rhizobium isolates R₁_, R₂, R₃, R₄_, R₅ and R₆. Among the Rhizobium isolates R₃ and R₅ of pea at 50% flowering stage had significant positive effective on nodulation, growth and nitrogen fixation of pea. There were high positive correlations among the number and dry weight of nodules, N content and uptake by shoot of pea.

Keywords: Rhizobium, Pea, Nodulation, Growth, Nitrogen fixation

Location: Jessore, Ishourdi (agro-ecological zone, AEZ 11), Faridpur, Rajbari (AEZ 12) and Barisal (AEZ 13) districts.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Pea

Objectives:

To characterize some Rhizobium strain isolated from root nodules and to assess their effectiveness in respect of nitrogen fixation in pea

Methodology:

A laboratory experiment was conducted to characterize six Rhizobium isolates recovered from nodules of pea collected from Jessore, Ishourdi (agro-ecological zone, AEZ 11), Faridpur, Rajbari (AEZ 12) and Barisal (AEZ 13) districts. Plants of pea were selected randomly from farmers’ field of the respected AEZs. Nodules separated from roots of pea were washed in fresh water and preserved in vial containing silica gel. Silica gel was used to soak the water of the nodules that might cause rotting of the nodules. The collected nodules were surface sterilized by exposing them to 95% alcohol for 5-10 sec. Then the nodules were immersed in 0.1% acidified mercuric chloride for 3-4 min. These nodules were then washed with six changes of sterile water. The nodules were then crushed and streaked on YEM agar medium. YEM agar medium contained the following constituents: K₂HPO₄ (0.5 g), MgSO_4.7H2_O (0.2 g), NaCl (0.2 g), CaCO₃ (0.2 g), FeCl₃.6H₂O (0.01 g), mannitol (10 g), yeast extract (0.4 g), agar (Difco, USA) (15 g), deionized water to 1 litre was used. The pH of the medium was adjusted to 7.0 with HCl solution. The medium was inoculated with the Rhizobium strains and incubated for one week. Colonies of the strains developed on the medium observed for their morphology and appearance. The YEM agar medium containing bromothymol blue indicator¹³ was used for identification of strains. The reaction of the rhizobial strains on this medium wasnoted every week. Fast-growing rhizobial strains produce acid in this medium, turning the medium yellow and slow growing rhizobia produce alkali, which turns the medium blue¹³^.

Source of Information: Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 45-48

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Inoculation treatments comprised of Rhizobium isolates R₁_, R₂, R₃, R₄_, R₅ and R₆. Among the Rhizobium isolates R₃ and R₅ of pea at 50% flowering stage had significant positive effective on nodulation, growth and nitrogen fixation of pea. There were high positive correlations among the number and dry weight of nodules, N content and uptake by shoot of pea.

Knowledge Management: Journal