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Comparative Study on Production of Antibody in Broilers Vaccinated with Four Lyophilized Newcastle Disease Vaccine

Kamrunnahar S
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Amin MM
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Taslima AHM
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Islam MA
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Paul NC
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

A comparative efficacy of four lyophilized Newcastle disease vaccine, namely, Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® in regard to the production of Himagglutination Inhibition (HI)- antibody was accomplished. For this, a total of 75 chicks were equally divided into five experimental groups such as, A, B, C, D and E. The HI antibody titers of group A vaccinated on 5 and 21 days of age with Medivac NDLaSota ® fluctuated among the Mean ± SD of 89.60 ± 33.05, 102.40 ± 33.05 and 192 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. As regards, group B administered with BCRDV® on the same days, the HI titers varied with the Mean ± SD of 83.20 ± 30.91, 102.40 ± 33.04 and 204.80 ± 66.09 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. When considered the HI antibody titers of group C inoculated with Izovac B1 Hitchner® on days 5 and 21 of age, HI titer exhibited the Mean ± SD of 80.00 ± 43.33, 96.00 ± 33.73 and 192.00 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. Elucidation of HI antibody titers of group D birds receiving Cevac Vitapest-L® on same days were recorded to be of Mean ± SD of 96.00 ± 33.73, 115.20 ± 26.98 and 320.00 ± 173.31 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. In birds of unvaccinated control group E, HI titers were found to be ranged from 32- 64, 16-32, 8-16, 4-8 and 2-4 with Mean ± SD of 48.00 ± 16.87, 24.00 ± 8.43, 11.20 ± 4.13, 5.20 ± 1.93 and 3.40 ± 0.97 on 3, 15, 17, 19 and 31 days of age respectively. Thus, it was found that birds of group A, B, C and D vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® induced slightly higher level of HI antibody titers than that of BCRDV. Maternally derived antibody (MDA) persisted to a minimal level until the age of chicks of day 17 and later on MDA declined.

Keywords: Vaccine, Antibody titer, Newcastle disease, Virus

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

1.To determine the maternally derived antibody of broiler chicks;

2.To determine the antibody titer in chicks following vaccination with Medivac ND LaSota® (LaSota strain), BCRDV® (F-strain), Izovac B1 Hitchner® (B1 strain) and Cevac Vitapest L® (LaSota strain),

3.To evaluate the comparative antibody production of the vaccines used.

Methodology:

Four Newcastle disease lyophilized (freeze-dried) vaccines were used in this study including (i) Medivac ND-LaSota® vaccine of LaSota strain (ii) Baby chick Ranikhet Disease vaccine (BCRDV®) of F (Asplin) strain (iii) Izovac B1 Hitchner® of B1 strain and (iv) Cevac vitapest-L® of LaSota strainThe antigens of strain F, Asplin, BCRDV used for Hemagglutination (HA) and HI test were collected from the repository of the Department of Microbiology and Hygiene, Bangladesh Agriculture University (BAU), Mymensingh. The virus was grown in 10-day-old embryonated chicken egg via allantoic sac (AS) route and allantoic fluid (AF) was collected used as antigen described by Cottral9.A total of 75 day-old chicks with the history of vaccination of parent stock against Newcastle disease (ND) were obtained for the experiment. After collection of blood at day 3 from 10 randomly selected birds, the birds were divided into 5 groups such as A, B, C, D and E and each group was reared separately from day 3. Group A, B, C and D were vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac vitapest-L® respectively at 5 days of age through intraocular route, and then boostered at 21 days age of birds, while group E was maintained as unvaccinated control. After collection of blood, serum samples were collected on day 3, 15, 17, 19 and 31 from all the groups Blood samples collected from chicken with anticoagulant were washed with PBS and centrifuged at 500 rpm for 5 mins. Discarding the supernatant, cRBC was collected and prepared a 2% and 0.5% cRBC in PBS for slide and micro HA test and HI test respectively. The unused cRBC suspension was stored at 4ºC until used.

Source of Information: Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 111-114

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In birds of unvaccinated control group E, HI titers were found to be ranged from 32- 64, 16-32, 8-16, 4-8 and 2-4 with Mean ± SD of 48.00 ± 16.87, 24.00 ± 8.43, 11.20 ± 4.13, 5.20 ± 1.93 and 3.40 ± 0.97 on 3, 15, 17, 19 and 31 days of age respectively. Thus, it was found that birds of group A, B, C and D vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® induced slightly higher level of HI antibody titers than that of BCRDV. Maternally derived antibody (MDA) persisted to a minimal level until the age of chicks of day 17 and later on MDA declined.

Knowledge Management: Journal