Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation and Identification of Uterine Microorganisms in Postpartum Dairy Cows

Azizunnesa
Assistant Professor, Department of Medicine and Surgery, Chittagong Veterinary and Animal Sciences University (CVASU),

S D Joy
MS student, Department of Medicine and Surgery, CVASU

M. O Faruk
Veterinary Surgeon, Hathazari, Chittagong, Department of Livestock , Bangladesh.

Abstract/ Executive Summary:

The aim of this study was to isolate and identify uterine microorganisms in postpartum cows and to observe the occurrence of uterine infection in crossbred and local cows. One hundred and nine samples, from both crossbred and local dairy cows were collected from Chittagong area. They were inseminated artificially (n=89) and naturally (n=20). Samples were collected at least 3 months after calving. Among the selected cows, 33 had different reproductive diseases and the rest were from apparently healthy cows. The crossbred dairy cows were more susceptible to reproductive diseases (19.27%) than the local ones. Uterine infection was higher (52%) in cows inseminated artificially than that of natural ones (21.21%). Nineteen different types of bacteria were isolated from both diseased and healthy animals, except Clostridium spp and Nocardia spp which were not found in diseased animal. Likewise, Bacillus spp and Salmonella spp were absent in healthy animals. Most prevalent bacteria in diseased animal were Staphylococcus spp and Streptococcus spp found in 33% and 27.3% of the cases respectively. In non-diseased cases, the highest number of samples was positive for Bacteroied spp (13.2%) followed by Streptococcus spp (10.4%). On average, the highest number of bacterial population was isolated from diseased samples and most of those bacteria are pathogenic

Keywords: Uterine infection, Identification, Postpartum, Dairy cows

Location: CVASU

Start Date: 00-06-2009

End Date: 00-12-2009

Program Area: Pest Management

Commodity: Cow

Objectives:

1.To know the occurrence of uterine infection in dairy cows

2.To identify the common bacterial population associated with uterine infection in cows.

Methodology:

The research was conducted in the Department of Medicine and Surgery, Chittagong Veterinary and Animal Sciences University (CVASU), Khulshi, Chittagong during the period from June, 2009 to December, 2009. The animals were selected from Chittagong metropolitan area. A total of 109 (n=109) cows including both crossbred (n=66) and local (n=43) were selected which crossed a minimum period of three months after parturition. They were in between 2 and 8.5 years of age, categorized as 7-8.5, 5-6 and 2-4 years old. The studied cows were also categorized according to parity. They were in different parity from 1st to 3rd and few cows were more than 3rd parity. A questionnaire was prepared and used to record information on age at birth, breed, parity, current physiological status and reproductive performance, recent major clinical illness, last calving date, nature of calving, insemination date and nature of insemination, diseases and treatment. Information in this study was recorded by face to face interviewing the owner and from the individual animal card, routine clinical case register, breeding register maintained at the dairy farm, record book of Artificial Insemination center etc. Clinical examination was also done to identify any abnormalities of cows.The cows were kept under traditional management systems. The housing was open or semi-intensive for most of the local cows. Some crossbred cows were reared in the intensive farming system. Both stall and group feeding were practiced round the year. The local cows were supplied with straw, roadside grass, bran and ad libitum water twice or thrice a day. Approximately, 8-10 kg roughage and 1.5-2 kg concentrate were provided to crossbred cows twice daily with free access to drinking water. Hand milking was practiced twice a day Prior to collection of samples, each cow was examined clinically. Body conformation, posture and gate, mucous membrane, respiration rate, pulse rate, temperature and any abnormalities, if present, were taken for each cow. Finally, thorough rectalpalpation was done to detect estrus and disease conditions of the cows Before collection of transcervical uterine sample, the perineal region of the cows were washed and cleaned properly with fresh water and potassium permanganate solution. Left handed rectal palpation was performed to collect uterine sample by using AI tube. A 10 ml syringe adjusted behind the glass made AI tube with rubber pipe was introduced into the uterus through cervix guided by rectal palpation. The collected sample was transferred to the vaccuotainer tube containing phosphate buffer saline (PBS) for aerobic culture and thio-glycolate broth for anaerobic bacterial culture. Paraffin oil was poured on to the surface of anaerobic sample to create an anaerobic condition. All these samples were preserved at -20 ^oC until cultureFor the growth of aerobic bacteria the samples were plated on nutrient agar, McConkey agar and mannitol Salt agar media and incubated at 37^oC for 48 hours. On the other hand for anaerobic bacterial growth, anaerobic incubator was used. In the case of anaerobic bacteria, nutrient agar and blood agar media were used. All the procedure was conducted at 37^oC temperature for 48 hours.The selected colonies of bacteria were identified on the basis of their colony characteristics, Gram stain, hemolytic properties and growth on selective media. After presumptive identification, the culture specific biochemical tests were performed All data obtained were computerized by MS Excel and subjected to standard statistical analysis. STATA/IC - 11 were done for p value. Differences were considered to be significant at 5% level of significance.

Source of Information: Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 19-23

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, the prevalence of uterine infection is higher in crossbred than local cows. Cows were inseminated artificially suffered more in uterine infection than inseminated naturally. Different pathogenic, non pathogenic, facultative, aerobic and anaerobic bacteria were identified from uterus in dairy cows. However, higher percentages of bacteria were found in diseased samples than non diseased samples. It also observed that older cows are most susceptible to different reproductive disorders and uterine infection than younger.

Knowledge Management: Journal