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Influence of Growth Regulators on Shoot Proliferation and Plantlet Production from Shoot Tips of Banana

S. Rehana
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh

M. S. Alam
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh

K. S. Islam
Intercooperation AFIP Project, Gulshan-2, Dhaka, Bangladesh

M. A. Samad
Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

Abstract/ Executive Summary:

The effect of BAP and IBA on in vitro regeneration of four banana cultivars viz. ‘Amritsagar’, ‘Seeded banana’, ‘Sabri’ and ‘Anajee’ was studied. The response of single shoot regeneration from shoot tips of four banana cultivars at different concentrations of BAP was found to be different. The cultivar ‘Amritsagar’ produced the highest percentage (60 %) of single shoot at 4.0 mg/l BAP within 10-15 days. The cultivar ‘Sabri’ and ‘Anajee’ produced lower percentage of single shoots. Rates of shoot multiplication of ‘Amritsagar’, ‘Sabri’, and ‘Anajee’ were 6-7 plantlets/explant, 2-4 plantlets/explant, and 2 plantlets/explant, respectively on medium containing 4.0 mg/l BAP after 30 days of culture. But in subsequent subculture, on the same medium, ‘Amritsagar’ produced the highest number of plantlets (9 plantlets/explant) within the same period of time. The best root formation in multiplied shoots of ‘Amritsagar’ was found on MS medium containing 2.0 mg/l IBA after 15 days of culture. All the in vitro cultured banana plantlets of ‘Amritsagar’ survived when weaned to ex vitro conditions on soil.

Keywords: Banana, Cultivar, In vitro, BAP, IBA, Micropropagation

Location: Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Banana

Objectives:

To assess the effect of different doses of BAP and IBA on in vitro regeneration and rooting of four commercially important varieties/cultivars of banana in Bangladesh.

Methodology:

The experimental materials such as Amritsagar (Genotype AAA), Sabri (Genotype AAA), Anajee (Genotype ABB), Seeded banana (Genotype BB) were collected from the neighboring villages of Bangladesh Agricultural University, Mymensingh and used as the source materials for obtaining meristem tip. The shoot tips were initially prepared by removing the outer layers of tissues from suckers with a clean knife. Then they were taken in a beaker and were sterilized with 70 % ethanol for one minute. Then they were washed 3 times with sterile distilled water and finally surface disinfected inside the laminar airflow cabinet with 0.1 % HgCl₂solution for 15 minutes followed by washing thrice with sterile distilled water. The explants were placed on MS medium supplemented with five concentrations of BAP (0, 2, 4, 6, 8 mg/l). Ten explants were cultured per treatment. All cultures were incubated at 25 ± 2°C. A 16-hour light period was maintained with a light intensity of 2000 lux for the growth and development of the cultures. The pH of the medium was adjusted to 5·8. The medium was sterilized by autoclaving at 15-psi pressure and 121°C temperature for 20 minutes. The materials were subcultured at 30 days interval in the same fresh medium to produce multiple shoots. Total numbers of shoots/explant and average length of shoots were recorded up to 4th subculture. The regenerated shoots were cultured in rooting medium consisting of MS basal salts supplemented with different concentrations of IBA (0, 1, 2, 3 and 4 mg/l). Days to root induction, number of root per explants and length of root were recorded 30 days after incubation in rooting medium. When the plantlets became 4-8 cm in length with 3-6 well-developed leaves and roots, the plantlets were removed from conical flasks. The roots were gently washed out with tap water. Plantlets were then individually transplanted to pots containing a mixture of ground soil and cowdung in the ratio of 1:1. Immediately after transplantation, the plants along with pots were covered with moist polythene bags to prevent desiccation. To reduce sudden shock, the pots were kept in the controlled environment in growth room for 7 days. Then the polythene bags were removed and the plantlets were kept in glasshouse. After 2 months when the plantlets attained a height of about 20-25 cm, they were transferred to the field.

Source of Information: Progress. Agric. 20(1 & 2) : 9 – 16, 2009 ISSN 1017-8139

Article Link (Online Journal): http://www.banglajol.info/index.php/PA/issue/view/1013

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present article describes a method of proliferation of shoot and formation of banana plantlets from shoot tip. This method has the potentiality to use as a tool of micropropagation of banana. Shoot tip derived plantlets are expected to be free from virus and other pathogens. However, further study is need for the confirmation of the in vitro shoots to be free from virus.

Knowledge Management: Journal