Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Antimicrobial Susceptibility of Vibrio Species Isolated from Brackish Water Shrimp Culture Environment

M. S. HOSSAIN
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

M. AKTARUZZAMAN
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

A. N. M. FAKHRUDDIN
Agrochemical and Environmental Research Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka-1349, Bangladesh.

M. J. UDDIN
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

S. H. RAHMAN
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

M. A. Z. CHOWDHURY
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

M. K. ALAM
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Abstract/ Executive Summary:

A comparative study regarding prevalence and antimicrobial susceptibility of Vibrio species in the black tiger shrimp (Penaeus monodon Fabricius) culture environment as well as market samples were analyzed. Total Vibrio counts were found to range up to 2.5 × 10³ cfu/gm and 60 cfu/gm in shrimp and Gher water samples, respectively. Results revealed that the total vibrio count were found to be higher in samples taking from the market shops in Dhaka city than Gher shrimp samples and the live shrimps were not contaminated with Vibrio species but dead shrimps were found contaminated, which were collected from shrimp Gher. In the antimicrobial susceptibility test, highest 28.57% resistance among total Vibrio isolates was observed to penicillin and cephalexin. Among the seven isolates of Vibrio species from Gher water sample one isolate displayed multi-drug resistance (MDR). The main reason of antibiotic resistance could be the indiscriminate application of antibiotics in shrimp farming and release of shrimp pond effluent to estuarine ecosystems or post harvest contamination of shrimps with the antibiotic resistant bacteria through the environment and human handling.

Keywords: Food safety, Vibrio species, Penaeus monodon, Prevalence, Antimicrobial resistance

Location: Kaliganj, Satkhira

Start Date: 01-07-2011

End Date: 31-12-2011

Program Area: Resource Development and Management

Commodity: Shrimp

Objectives:

To determine the prevalence of drug resistant Vibrio species in cultured black tiger shrimp and shrimp pond environment.

Methodology:

The black tiger shrimp and Gher water samples were collected from Kaliganj Upazilla in Satkhira district of Bangladesh where most of the shrimp farming is carried out. The study area lies between the latitudes 22^o27^o34N and longitudes 89^o01´37E. The samples were collected in July, 2011. Besides these, five shrimp samples were collected from the different markets of Savar and Dhaka city to compare the prevalence of pathogenic bacteria with the shrimp Gher samples. The shrimp samples were collected individually in pre-sterilized polyethylene bags and water samples in pre-sterilized container and brought to the laboratory as soon as possible for the analysis. To avoid further contamination, during transportation from the source to the laboratory, samples were kept in insulated box with ice to maintain the temperature around 4 to 6^oC.Two hundred fifty gram of shrimp samples were blended aseptically. From the blending portion, 10 gm sample and 90 ml sterile normal saline (0.9% w/v, NaCl) solution were taken in a conical flask to make a homogenous suspension. Samples were serially diluted and thoroughly mixed using Vortex-mixture and analyzed for microbial content. Thiosulphate citrate bile salt sucrose (TCBS) agar was used for enumeration of total Vibrio bacteria. The TCBS agar has been melted, brought to 45°C and was poured into sterile petridishes. After solidifying and inoculation, plates were incubated at 35°C for 24 to 48 hours. Distinctive yellow and green colonies on agar plates were picked and streaked on new TCBS agar plates several times to purify bacterial isolates until pure cultures of isolates were obtained. Gher water samples were also analyzed in an identical manner. A total of 12 antibiotic discs comprising of streptomycin 10 µg, erythromycin 15 µg, chloramphenicol 30 µg, ciprofloxacin 5 µg, penicillin 10 µg, norfloxacin 10 µg, ampicillin 10 µg, kanamycin 30 µg, amoxicillin 10µg, azithromycin 15 µg, cephalexim 30 µg and sulphamethoxazole 25 µg were used. Within 15 min of the application of the discs, the plates were inverted and incubated at 37°C. After 16-18 hr of incubation, the plates were examined, and the diameters of the zones of complete inhibition to the nearest whole millimeter were measured.

Source of Information: Journal of Bangladesh Academy of Sciences, Vol. 36, No. 2, 213-220, 2012

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the study the total vibrio count were found to be higher in samples taking from the market shops in Dhaka city than Gher shrimp samples and the live shrimps were not contaminated with Vibrio species but dead shrimps were found contaminated, which were collected from shrimp Gher. In the antimicrobial susceptibility test, highest 28.57% resistance among total Vibrio isolates was observed to penicillin and cephalexin. In the present study, among the seven selected isolates, one isolate displayed multi-drug resistance to more than three antibiotics. The incidence of Vibrio species in shrimps and their drug resistance pattern in this study demands immediate need for paying attention in this area.

Knowledge Management: Journal