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Estimation of Effect of Rhizobium Inoculant, Phosphorus and Sulphur on the Growth and Yield of Blackgram

S.M.A. Shahin
Department of Soil science, Bangladesh Agricultural University, Mymensingh – 2202

M.H.Shohel
Exim Bank, Agricultural University, Bangladesh, Chapainawaabganj

M. Hassan
Department of Soil science, Bangladesh Agricultural University, Mymensingh – 2202

M.A. Tarafder
Soil science Division, BINA, Mymensingh

M.H. Mian
Department of Soil science, Bangladesh Agricultural University, Mymensingh – 2202

Abstract/ Executive Summary:

An experiment was conducted at the experimental field of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during November 2013 to January 2014 to study the effect of P, S and Bradyrhizobium inoculant on nodulation, yield and yield contributing characters of blackgram. Eight treatments were: Control, Inoculum, P, P+ lnoculum, S, S + Inoculum, P + S, and P + S + Inoculum. The experiment was laid out in a Randomized Complete Block design with three replications. The rates of P, S and Bradyrhizobium inoculant were 40 kg P ha^-1 15 kg S ha^-1 and 15 g inoculant kg-¹ seed, respectively. TSP was used as a source of P and gypsum for S. Basal application of 40 kg K ha^-1 and 2 kg Zn ha^-1 was made to all plots. At 30 and 55 DAS, 5 plants were uprooted from each plot for recording data on nodulation and growth characteristics. The crop was harvested at maturity at 90 (DAS). The results indicated that combined application of chemical and biofertilizer performed better than single application of fertilizer in respect of plant height (15.50 cm), dry matter plant^-1 at 30 (DAS) (310.3 mg) and 55 DAS (4487mg); and number of nodules plant^-1at both 30 DAS (14.67) and 55 DAS (27.00).Yield and yield contributing characters like number of seed weight plant^-1, seed yield, stover yield were significantly influenced by biofertilizer application. Thus Bradyrhizobjum inoculation was found essential for blackgram cultivation at BINA Farm and an application of P and S further increased the yield.

Keywords: Rhizobium inoculant, Phosphorus, Sulphur, Blackgram

Location: Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

Start Date: 00-11-2013

End Date: 00-01-2014

Program Area: Crop-Soil-Water Management

Commodity: Black gram

Objectives:

1. To evaluate the performance of Rhizobium inoculants on the growth and yield of black gram; and investigate the combined effect of Rhizobium inoculants with P and S fertilizer.

Methodology:

The experiment was conducted at farm of Bangladesh Institute of Nuclear Agriculture (BINA) head quarter farm, Mymensingh; under Old Brahmaputra Floodplain (AEZ 9) during November to January 2013-2014. The crop used in this study was Blackgram (Vigna mungo). There were eight treatments in different combinations of biofertilizer ,P,and S replicated thrice in randomized complete block design.Details of treatment combination. The Rhizobium strain was inoculated into yeast mannitol broth medium containing 250 ml Erlenmeyer flask under laminar flow cabinet. The inoculated flask was then placed on an electric shaker for seven days for optimal growth of Rhizobial cell in the broth culture. After seven days, the bacterial culture was tested its purity and growth. The Rhizobial cell was counted through plate count method. The population was maintained above 10 cells per ml broth culture. The population of Rhizobium cells were 1.9x10⁹, 2.0x10⁹ and 2.1x10⁹ per ml broth culture respectively. All the chemical fertilizers except nitrogenous fertilizer were mixed with soil.The rates of P, S and Bradyrhizobium inoculant were 40 kg P ha^-1 15 kg S ha^-1 and 15 g inoculant kg^-1 seed, respectively. TSP was used as a source of P and gypsum for S. Basal application of 40 kg K ha^-1 and 2 kg Zn ha^-1 was made to all plots. Five plant samples were collected from the field at 35 and 55 days of sowing and at harvest, from each plot, at random and were carefully uprooted with the help of a nirani cuttin into a soil block in the f so that no nodules were left in the soil. The soil blocks were kept for sometime in water buckets to soak before gently washingout the soil from the roots. The roots were then finally washed carefully with water. The nodules from root of each plant were collected. The length of the roots and shoots of each plant were recorded. Initial soil samples were collected and analyzed at soil science laboratory BINA.

Source of Information: J. Agrofor. Environ. 8 (2): 27-29, 2014; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The results indicated that combined application of chemical and biofertilizer performed better than single application of fertilizer in respect of plant height (15.50 cm), dry matter plant^-1 at 30 (DAS) (310.3 mg) and 55 DAS (4487mg); and number of nodules plant^-1at both 30 DAS (14.67) and 55 DAS (27.00).Yield and yield contributing characters like number of seed weight plant^-1, seed yield, stover yield were significantly influenced by biofertilizer application. Thus Bradyrhizobjum inoculation was found essential for blackgram cultivation at BINA Farm and an application of P and S further increased the yield.

Knowledge Management: Journal