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Effect of Indole-3-acetic Acid (iaa) on Biochemical Responses of Cowpea (vigna Unguiculata (l.) Walp) Var. BARI Fellon-1

K. Taslimaa
Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

F. Hossaina
Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

U. Arab
Institute of Food Science & Technology Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.

Abstract/ Executive Summary:

The biochemical responses of Cowpea to IAA treatments (foliar spray) with different concentrations (20 ppm, 40 ppm, 60 ppm and 80 ppm) were studied. Biochemical parameters such as leaf pigments, moisture, ash, crude fiber, calorie, protein, fat, carbohydrate and nitrogen free extract (NFE) contents in seeds of Cowpea were studied in the experiment. Different biochemical parameters responded differently following IAA treatments. Highest protein content (22.7%) and ash content (4.2%) were recorded from the seeds of the plants treated with 80 ppm IAA. Highest carbohydrate content (60.8%) and calorie content were recorded from the seeds of the plants treated with 60 ppm IAA. Moisture content was decreased due to all IAA treatments. Fat, crude fiber and NFE content of seeds also found to be varied with different treatments. Out of all the treatments, the best stimulation was found for 60 ppm IAA treatment. The results of the present work also indicate that an appropriate concentration of IAA can be fruitfully utilized to produce better quality of seeds and leaf pigments of Cowpea.

Keywords: Auxins, Biochemical response, IAA treatment, Cowpea

Location: Jahangirnagar University

Start Date: 00-12-2001

End Date: 00-04-2002

Program Area: Seed Technology

Commodity: Cowpea

Objectives:

To study the effect on biochemical changes of seed of Cowpea using different concentrations of IAA as well as to screen out the better concentration of IAA treatment

Methodology:

The field experiment was conducted in the experimental field of Botanical garden of Botany Department, Jahangirnagar University, Savar, Dhaka, during the period from December, 2001 to April, 2002. Seeds of Cowpea were collected from Agricultural University, Shere-a-bangla nagar, Dhaka which were used as plant material. The field was prepared with proper precautions and divided into 15 plots of (3.15m x 2.15m = 7.08 m2) having footpaths of 20 cm in between. After 40 days of emergence of the seedling a first spraying treatment was made and that was followed by second spraying after 15 days. Treatment schedule of IAA is given in Table I.. After the end of the field experiment, the biochemical analysis of the seeds of Cowpea were carried out at the plant physiology and biochemistry laboratory of Botany Department of Jahangirnagar University and partlyin Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka. Differently treated plants were separately harvested and seeds were collected from the plants for different parameters. Ten randomly selected plants were used from each treatment for collecting data. Chlorophyll a and b content of leaves were determined for each treatment from the age of 15 days after spray (DAS) at an interval of 10 days up to 35 DAS using UV spectrophotometer (Spectronic Unicam, England) in BCSIR. Specific absorption co-efficient method of Mckinney (1940) and the formula of Machlachalan and Zalik (1963) were used to determine the amount of chlorophyll-a and Chlorophyll-b. Quantitative determination of ash, crude fibre, protein, fat and carbohydrate content of seeds were determined following AOAC (1985) protocolStatistical analysis Data collected on different parameters were statistically analyzed and treatment means were compared by Least Significant Difference Test (LSD Test). The analysis of variance (ANOVA) was conducted following the procedure laid down by Gomez and Gomez (1984) to calculate the differences between the treatments. Data were also analyzed using SPS program .

Source of Information: Bangladesh J. Sci. Ind. Res. 46(1), 77-82, 2011

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From this study it can be concluded that different biochemical parameters responded differently following IAA treatments. Chlorophyll a, b contents were significantly influenced by different IAA treatments. Highest protein content (22.8%) and ash content (4.2%) were recorded from the seeds of the plants treated with 80 ppm IAA. Highest carbohydrate content (60.8%) and calorie content were recorded from the seeds of the plants treated with 60 ppm IAA. It was found that the moisture content decreased due to all treatments. Fat, crude fiber and NFE content of seeds also found to be varied with different treatments. The results of the present work also indicate that an appropriate concentration of IAA can be fruitfully utilized to produce better quality of seeds of Cowpea plants .

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