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Effects of Gibberellic Acid and Kn on Seed Germination and Accumulation of Na+ and K+ in the Seedlings of Triticale-i Under Salinity Stress

RIFAT SAMAD
Department of Botany, University of Dhaka, Dhaka

J L KARMOKER
Department of Botany, University of Dhaka, Dhaka

Abstract/ Executive Summary:

Effects of GA and Kn on seed germination and accumulation of Na+ and K+ in the seedlings of Triticale cultivar was investigated under salinity stress. Salinity decreased the rate of seed germination and ion accumulation in the seedlings but both GA and Kn stimulated these processes under stress. Salinity increased the accumulation of Na+ in radicles and plumules with concomitant inhibition of that of K+, GA and Kn, on the other hand, reduced the rate of Na+ accumulation and increased that of K+ in the seedlings. The interrelationship between the effect of salinity and growth regulators on seed germination and the accumulation of Na+ and K+ in the seedlings is discussed.

Keywords: Salinity, Germination, Ion accumulation, Triticale

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Start Date:

End Date:

Program Area: Knowledge Management

Commodity: Triticale

Objectives:

The effect of GA and Kn on seed germination and accumulation of Na+ and K+ in the developing seedlings under salinity stress is reported.

Methodology:

The seeds of Triticale (Triticosecale Wittmack) were obtained through the courtesy of Bangladesh Agriculture Research Institute, Gazipur. Methods of germination of seeds: The seeds were surface sterilized in 5.25% sodium hypochlorite solution for two minutes. Seeds were then rinsed seven times in tap water followed by washing four times in distilled water. The sterilized seeds dipped in distilled water were aerated for 30 minutes with an air compressor. Thirty such seeds were placed on Whatman filter paper No.1 contained in a Petri dish (11 cm diam.). Three replicates were used for each treatment. Filter paper was soaked with 6 ml of NaCl solution at concentrations of 25, 50, 100, 200, 300 and 400 mM each prepared in 0.1 mM CaSO₄ ± selected concentration of GA and Kn and the Petri dish was covered. In case of control, the filter paper was soaked with 6 ml of 0.1 mM CaSO₄_. The seeds were allowed to germinate in the dark at 28^oC ±1^oC/22^oC ±1^oC day/night temperature. However, 3 ml of test solution was added after 72 h from the time of sowing. Seeds were considered to be germinated when radicles and plumules could be clearly distinguished. Seed germination activity was recorded at 48, 72 and 96 hrs from the time of sowing. Extraction and analysis of Na+ and K+ in radicles and plumules: Radicles and plumules were separated from cotyledons at 48, 72 and 96 hrs from the date of sowing. Each set of radicles or plumules was washed in two changes of 0.1mM CaSO4 contained in a small beaker (50 ml each) for two minutes to remove free space ions and were dried in an oven (Memmert, M-3132, Germany) at 75^oC for 72 hrs to a constant weight. Na+ and K+ were extracted by hot water following Karmoker and Van Steveninck (1978). After necessary dilution Na+ and K+ in the extract was measured using a flame photometer (Jenway PEP-7, UK) at a wavelength of 767 and 589 nm, respectively.

Source of Information: Bangladesh J. Bot. 41(2): 123-129, 2012 (December)

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Achievement/Findings:

In conclusion, inhibition of germination by salinity might be due to the salinity-induced increase in accumulation of Na+ with concomitant inhibition of that of K+ in the radicles and plumules of germinating embryo of Triticale. The nullification of an inhibitory effect of salinity on germination leading to a stimulation of that following interaction of GA and Kn might be due to GA- and Kn-induced decrease in Na+ accumulation with concomitant increase in the uptake of extruded K+ in germinating embryo of Triticale.

Knowledge Management: Journal