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Effect of Growth Regulator on Regeneration of Two Sweet Potatoes (ipomoea Batatas L.)

M. M. Kamal
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The experiment was conducted to investigate the effect of various concentrations of different growth regulators on the aspect of callus induction along with shoot regeneration and root formation of two sweet potato varieties viz. BARI 6 and BARI 7. For callus induction, MS medium was used supplemented with different concentrations of 2,4–D (0, 0.1, 0.5 and 1.0 mg L^–1) and Kinetin (0, 0.1, 0.5 and 1.0 mg L^–1)). Different concentrations of BAP (0, 1.5, 2.5, 3.5 and 4.5 mg L^–1) were used for shoot regeneration while different concentrations of NAA (0, 0.1, 0.5, 1.0 and 1.5) with a constant level of BAP @ 2.0 were also used for root development. All the studied parameters were significantly influenced by the effect of sweet potato varieties and various concentrations of growth regulators. The highest percentage (91.67%) of callus induction was observed in 0.5 mg L^–1 2,4–D + 0.5 mg L^–1 kinetin with the variety BARI 6 within a minimum number of days (5.50 days) while similar effect also showed the higher weight of callus at 15 (4.65 g) and 35 (6.12 g) days of culture. Performance study of BAP concentration regarding two potato varieties were significant among the shoot characters where 2.5 mg L^–1 BAP treated callus of BARI–6 obtained the maximum regeneration of shoot (85.00%) with an average requiring minimum time (6.83 days) while maximum leaves shoot^–1 (12.17) and longest shoot (5.50 cm) were also obtained. Percentage of root formation (73.33%) had also maximum in BARI–6 with an average requiring least time (6.83 days) while it was treated by 1.0 mg L^–1 NAA which treatment also showed the longest root (10.83 cm).So, it is clear that the sweet potato variety BARI–6 showed superiority than BARI–7 while 0.5 mg L^–1 2,4–D + 0.5 mg L^–1 kinetin was the best combination for callus induction, 2.5 mg L^–1 BAP for shoot formation and 1.0 mg L^–1 NAA for root regeneration in the present study.

Keywords: BAP, BARI, Callus, Growth regulators viz. 2, 4–D, Kinetin, NAA, Shoot, Sweet potato, Root

Location: USDA Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh

Start Date: 00-05-2010

End Date: 00-09-2010

Program Area: Variety and Species

Commodity: Sweet potato

Objectives:

1. To study the effect of various concentration of growth regulator on the aspect of callus induction, shoot regeneration and root formation of BARI released two sweet potato varieties.

Methodology:

The research program reported here was carried out during the period from May to September 2010 in the USDA Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh. Two sweet potato varieties viz. BARI–6 and BARI–7 were used as experimental materials which were collected from the Bangladesh Agricultural Research Institute, Gazipur-1701. Leaf explant of sweet potato were used for callus induction on MS medium supplemented with different concentrations of 2,4–D (0.0. 0.1, 0.5 and 1.0 mg L^–1) in combinations with different concentrations of Kinetin (0.0,0.1, 0.5 and 1.0 mg L^–1). After callus induction, calli were used for shoot regeneration in different concentrations of BAP (0.0. 1.5, 2.5, 3.5 and 4.5 mg L^–1). The regenerated shoot were rooted on MS medium supplemented with different concentrations of NAA (0.0. 0.1, 0.5 and 1.0 mg L^–1) in combination with a constant level of BAP (2.0 mg L^–1). To investigate the effect of different treatments of growth regulators on callus induction, shoot regeneration and root formation, the data were recorded on days to callus induction, callus induction (%), weight of callus at 15 and 35 days of culture, shoot regeneration (%), days to shoot initiation, number of leaves shoot^–1, shoot length, percent shoot sowing root, days to root and length of root. The experiment was laid out in a completely randomized design (CRD) with three replications. The collected data were analyzed statistically and means were adjudged by DMRT at 5% level of probability.

Source of Information: J. Environ. Sci. & Natural Resources, 7(1): 113 – 116, 2014; ISSN 1999-7361

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Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It could be concluded that the 0.5 mg L^–1 2,4–D + 0.5 mg L^–1 kinetin for callus induction, 2.5 mg L^–1 BAP for shoot regeneration and 1.0 mg L^–1 NAA in combination with 2.0 mg L^–1 BAP for root formation were optimum concentration in the present study for both the sweet potato varieties. However, sweet potato variety BARI 6 showed the better performance than BARI 7 with the assigned growth regulators in respects of the studied all characteristics.

Knowledge Management: Journal