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Research Detail

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A. Khatun
Planning and Evaluation Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. A. H. Bhuiyan
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. M. Ud-deen
Department of Crop Science and Technology, University of Rajshahi, Rajshahi-6205, Bangladesh

Laboratory experiments were carried out to determine the effects of harvesting stages on the seed quality of chickpea. Chickpea seed of three varieties (BARI Chola-5, BARI Chola-6 and BARI Chola-8) were collected at three harvestings times i.e. from three different times at 7 days interval prior to harvesting, viz. i) when the pods were yellowish with a few yellow greens (H1 stage), ii) when most of the pods were light brown with a few yellow (H2 stage), and iii) when all the pods were completely brown and dry (H3 stage). Significant variation was observed in three varieties of chickpea for all the parameters studied except vigour-I. The highest germination percentage, root length, shoot length, root plus shoot length and vigour-II was observed in BARI Chola-5 which was significantly higher over BARI Chola-8. Dry weight was found to be significantly higher in BARI Chola-8 which was identical to BARI Chola-6. Moisture content was significantly higher in BARI Chola-8. Seed collected at the stage when most of the pods were light brown with a few yellow (H2 stage) recorded the highest germination percentage, dry weight, vigour-I and root length. Pods (H1 stage) gave the highest moisture content in seeds. BARI Chola-5 seeds recorded the highest germination at H2 stage. Interaction effects of varieties and harvesting stage had non-significant effect on germination percentage, vigour-I, shoot length and vigour-II in both the years.

  Chickpea, Harvesting stage, Storage, Vigour, Moisture, Germination
  Seed Technology Division Laboratory in the Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur, Bangladesh
  00-00-2004
  00-00-2005
  Crop-Soil-Water Management
  Chickpea

The investigation was carried out to find out the right time of harvest to ensure seed quality of chickpea.

Laboratory experiments were carried out at Seed Technology Division Laboratory in the Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur, Bangladesh during the period from August to September 2004 and 2005 to determine the effects of harvesting stages on seed quality of chickpea under laboratory conditions. Previously chickpea seeds of three varieties viz. BARI Chola-5, BARI Chola- 6 and BARI Chola-8 were collected from Pulses Research Centre experimental field of BARI, Bangladesh in rabi seasons of 2003-04 considering three different times of harvest at 7 days interval, viz. i) when the pods were yellowing with a few yellow greens (H1 stage), ii) when most of the pods were light brown with a few yellow (H2 stage), and iii) when all the pods were completely brown and dry (H3 stage). The collected seeds were preserved in the earthen pot. Before setting up the field experiment, laboratory experiment was carried out for recording the seed quality data. Laboratorial experiments were conducted in Complete Randomized Design with the above seeds of chickpea. The following quality parameters (moisture %, germination %) and seed vigour contributing characters were recorded: Determination of moisture content: The moisture content of seed samples were determined by ISTA, 1976. Grinded (10 g) seed samples each of chickpea were taken into moisture cup and put into a pre-heated oven at temperature of 103 ± 2OC for one hour. Three replicates were taken. After cooling, the weight of the container with its cover and contents were taken. The seed samples were cooled in desiccators and weighed to work out the percent moisture content of the grains. The seed moisture content was determined by dry weight basis. Determination of germination test Germinations were carried out according to ISTA rule 1976. For each treatment, 100 seeds were put into petri dishes. Four replicates were used. The petri dishes were put up on a laboratory table at room temperature (25±2OC). After ten days, normal, abnormal and diseased seeds were counted. Measurement of root and shoot length: After ten days, ten plants were randomly selected for study, taking from each replicate of each treatment. The seedlings were cut into root and shoot parts. They were measured (in cm) and the mean value was computed. Determination of fresh and dry weight of seedling: After measuring the root and shoot length as described above, fresh weight of seedlings was recorded. Then the root and shoot were put into paper packet separately, and placed into the preheated oven (70OC) for 48 hours. After cooling in desiccators, the dry weight was taken. Determination of seed vigour: Seedling vigour was calculated based on the following formulae (Reddy and Khan, 2001): Vigour index-I: Percent germination x total dry weight of seedling. Vigour index-II: Percent germination x length of seedling. The data for different characters were compiled and tabulated in proper form and then subjected to statistical analysis following a computer IRRISTAT package programme adjusted the means. The correlation co-efficient were done for different variables wherever needed.

  Bangladesh J. Sci. Ind. Res. 44(3), 303-310 2009
  
Funding Source:
  

The highest germination percentage, root length, shoot length, root plus shoot length and vigour-II was observed in BARI Chola-5 which was significantly higher over BARI Chola-8 and the lowest in BARI Chola-8. Seed collected at the stage when most of the pods were light brown with a few yellow (H2 stage) recorded the highest germination percentage, dry weight, vigour-I and root length. Pods collected at the stage of yellow with a few yellow green (H1 stage) gave the highest moisture content in seeds. BARI Chola-5 seeds collected (H2 stage) recorded the highest germination.

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