Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Clonal Propagation of Scoparia Dulcis L., a Perennial Medicinal Herb

A. K. M. Sayeed Hassan
Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh.

Laila Shamroze Bari
Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh.

Rebeka Sultana
Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh.

Nadira Begum
Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh.

Rahima Khatun
Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh.

Abstract/ Executive Summary:

An efficient protocol was established for in vitro clonal propagation of the perennial medicinal herb Scoparia dulcis L. (Family. Scrophulariaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.1 mg/l BAP, in which 94% of the explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 16 shoots per culture. The half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA the highest percentage (85.20) and maximum number (13.40) of roots were initiated within four weeks of culture. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%.

Keywords: Scoparia dulcis, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization, IAA (indoleacetic acid), IBA(indolebutanoic acid), NAA (α-naphthaleneacetic acid), BAP (benzylamino purine)

Location: Biological Research Division in Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Non-commodity: Performance

Objectives:

The present study was, therefore, undertaken to develop a protocol for mass clonal propagation by using shoot tips and nodal explants of this important medicinal herb through in vitro culture.

Methodology:

The experiment was conducted at Biological Research Division in Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka. Healthy and profusely growing vine of Scoparia dulcis L. was collected from BCSIR campus, Dhaka and used as source of explants. Shoot tips and nodal explants with a single axillary bud were used for this purpose. The explants were washed thoroughly under running tap water, pre-soaked in liquid detergent for about 30 min, wiped with cotton and dipped in 70% (v/v) ethanol for 1 min. They were then surface-sterilized with 0.1% (w/v) mercuric chloride for 5 min, followed by five rinses with sterile distilled water in front of a laminar air flow cabinet. The surface-sterilized explants were sized to 1-1.5 cm length containing a single node with an axillary bud or a shoot tip with an apical bud. The explants were placed vertically on the culture medium. The new shoots induced from the in vitro cultures were further used as an explants for adventitious shoot regeneration. MS (Murashige and Skoog, 1962) basal medium was used for shoot proliferation and adventitious shoot regeneration and half strength MS was used for in vitro rooting. All the media were supplemented with 30 g/l sucrose, 7 g/l agar (Difco) and dispensed into 15x150 mm culture tubes and 250 ml conical flasks. The pH of the media was adjusted to 5.8 before autoclaving at 121^OC for 20 min. The cultures were incubated for a 16 h photoperiod at 24 ± 2^OC under a fluorescent light. Shoot proliferation from shoot tips and nodal explants was obtained in two separate sets of experiments. In the first experiment 0-2.5 mg/l BAP and 0-2.5 mg/l Kn were incorporated into MS to select the best cytokinin for the response of shoot induction. In the second set, combination of BAP (0.1-0.3 mg/l)-NAA (0.05-0.1 mg/l) and BAP (0.1-0.3 mg/l)- IAA (0.05-0.1 mg/l) were assessed for shoot multiplication. Number of new shoot proliferation of each culture was recorded after every week of inoculation. For in vitro rooting, individual shoots (3-5 cm) were excised from the proliferated shoot cultures and implanted into half strength MS with different concentrations and combinations of NAA, IBA and IAA. The rooted plantlets were taken out from the culture tubes, washed to remove agar gel adhered to the roots and transplanted to plastic pots with soil and compost (1: 1) for hardening. The plantlets were kept in a polychamber at 80% relative humidity, 32 ± 2^OC under a 12 h photoperiod for acclimation. Established plants were transplanted in earthen pots under natural conditions and the survival rate was recorded.

Source of Information: Bangladesh J. Sci. Ind. Res. 44(3), 341-346, 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

None of the plantlets survived when directly transferred from rooting medium to the pot under natural conditions. About 85 percent of the transplanted plants of Scoparia dulcis survived if the plantlets in the rooting medium were kept in normal room temperature for seven days before transplantation in pots and reared for three weeks. The plantlets were reared under semi-controlled temperature (30±2^OC) and light (2000 lux) in a chamber with 80 percent humidity. During this period of acclimation shoots elongated, leaves expanded and turned deep green and healthier. After three weeks, plants were transferred to an open place and gradually acclimated to outdoor conditions, where 80 percent plants survived. The technique described here appears to be readily adaptable for large scale clonal propagation and plantation for sustainable use in the industry. Moreover, by standardizing the protocols for clonal propagation of selected elite plants, it is possible to achieve a tenfold increase in the products per unit area of cultivation. Clonally propagated plants would also have identical phytochemical profiles. Likewise it could be possible to propagate important medicinal plants for cultivation and sustainable use and consequently to conserve them from their extinction.

Knowledge Management: Journal