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In Vitro Plant Regeneration in Banana (musa Sp.) Cv. Sabri

Amdadul Huq
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.

Shahina Akter
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.

Shahina Islam
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.

Salim Khan
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh.

Abstract/ Executive Summary:

The establishment of a micro-propagation protocol for banana (Musa sp.) cv. Sabri, was carried out using meristematic stem cuttings explant. MS medium supplemented with BAP singly or in combination with auxin, IAA and coconut water was used for this purpose. Highest percentage of shoot regeneration (90%) and maximum number of shoots (10) per explant were observed when cultured on MS + 4.0 mg/1 BAP + 2.0 mg/1 IAA + 13% (v/v) coconut water. Best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 IBA. The regenerated healthy rooted plant lets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Through this method, complete micro-propagated plant lets were obtained within three months.

Keywords: Investigate,In vitro,Plant regeneration, Banana

Location: BCSIR, Dhanmondi, Dhaka.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Banana

Objectives:

To investigate the In vitro plant regeneration in Banana (Musa sp.) cv. sabri

Methodology:

The experiment was conducted at Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka. Sword suckers of banana were collected from the experimental field of BCSIR. Shoot tips were prepared by removing a number of outer leaf sheaths from the suckers. The explants were surface sterilized with soft detergent for three times followed by washing with a few drops of Tween 20 and thoroughly washed in running tap water for 20-25 minutes. Then the explants were surface sterilized with 0.1% mercuric chloride(HgCl₂) for 8 minutes. The sterilized explants were then rinsed 4-5 times with sterile distilled water inside the clean bench to remove all traces of HgCl₂. The sterilized explants were excised in the laminar airflow cabinet aseptically using a fine sterile forcep and scalpel. The excised explants were then inoculated on MS medium supplemented with BAP (1.0, 2.0, 3.0, 4.0, 5.0 mg/l) alone or in combination with IAA (0.5, 1.0, 2.0 mg/l) and coconut water (10%, 13%, 15%) for shoot regeneration and multiplication. The pH of the medium was adjusted to 5.8 ± 0.1. The cultures were maintained at the temperature set on 25 ± 2oC with a light intensity of 2000 lux from fluorescent lamps. The maintained photoperiod was 18 hours light and 6 hours dark (18 L/6 D). The established cultures on shoot induction medium were routinely transferred after every 3-4 weeks. The percentage of explants induced shoot, days to shoot initiation and number of shoots per explant have been recorded after three weeks of culture. In vitro shoots of banana were cultured on ½ MS medium supplemented with different concentrations of IBA (0.1, 0.3, 0.5, 1.0, 2.0 mg/l) and NAA (0.1, 0.3, 0.5, 1.0, 2.0 mg/l) for root initiation. The well rooted plantlets were then kept in room temperature for 2-3 days and transferred to plastic pot containing garden soil and compost in ratio of 2:1 and moist them adequately for proper hardening.

Source of Information: Bangladesh J. Sci. Ind. Res. 47(2), 143-146, 2012

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Highest percentage of shoot regeneration (90%) and maximum number of shoots (10) per explant were observed when cultured on MS + 4.0 mg/1 BAP + 2.0 mg/1 IAA + 13% (v/v) coconut water. Best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 IBA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Through this method, complete micro-propagated plantlets were obtained within three months.

Knowledge Management: Journal