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Effect of Clove Oil as an Anesthetic on Labeo Rohita (hamilton)

S. M. Farid
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

M. M. Rahman
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh

K.K. Shirin
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

N. N. Nur
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

An experiment was conducted in the Wet Laboratory, Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh to study the efficacy of clove oil as an anaesthetic for Labeo rohita (Hamilton). Clove oil were used at different doses (0.01, 0.025, 0.05, 0.1, 0.2 and 0.3ml/l) on different size groups (4-6 cm, 9-11 cm, 14-16 cm, 19-21 cm.) of fish. During the experimental period water temperature was 25 to 28°C. The highest 8.35 minutes and lowest 0.48 minutes induction times of clove oil were recorded at the dose of 0.01 ml/l and 0.3 ml/l, respectively for all size classes. Induction times were decreasing with the increasing doses of clove oil. The highest 19.50 minutes and lowest 1.78 minutes recovery times of clove oil were recorded at the dose of 0.3 ml/1 and 0.01 ml/l, respectively for Labeo rohita of all size groups.

Keywords: Clove oil, Labeo rohita, Anaesthetic, Induction time

Location: Wet Laboratory, Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh

Start Date: 00-02-2007

End Date: 00-12-2007

Program Area: Animal Health and Management

Commodity: Carp fish

Objectives:

1. To assess the effectiveness of clove oil as an anaesthetic using water as solvents on Labeo rohita of different size groups.

Methodology:

The experiment was conducted for a period of 10 months in 6 Aquaria (33L) which were located in the wet laboratory at the south west corner of the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. All the aquaria were rectangular in shape and possessed similar size (45 x 25 x 30 cm). The experimental fishes were collected from the Fish Seed Multiplication Farm, Isharganj, Mymensingh and transported to the wet laboratory of the Department of Aquaculture, Bangladesh Agricultural University, Mymensingh by poly-ethylene bag with oxygen. The fishes were kept in a number of glass aquaria in the laboratory for a period of seven days at room temperature to acclimatize with the new environment and also with the regular feeding. Total length of the collected fishes were taken and catagorized in to four size groups according to the basis of their length. At first any leakage of each aquarium was checked up. The aquaria were cleaned with the help of common salt and were sponged thoroughly and water was scooped out from the aquaria. The aquaria were rewashed and filled almost up to the top level and kept for a day. The water of the aquaria was scooped out in the following day. The aquaria were again washed with the help of common salt to destroy the micro-organisms or other harmful elements. Then the aquaria were sponged and were filled with tap water and scooped out and then dried. Aquaria were placed on the iron made self in a row and filled with fresh water. One aerator (bubble contact aerator) was set up in each of the five aquaria arranged for static system. Prior to weighing fish were caught with a fine mesh scoop net and their group wise length and weight were recorded to the nearest (0.1) centimeter and nearest (0.1) gram respectively. The total length (cm) and weight (g) of the individual fish were carefully recorded. A wooden measuring board was employed for measuring the length. The total body weight of individual fish was determined by a sensitive electric balance and the fishes were kept in a number of glass aquaria in the laboratory for a period of seven days to acclimatize the fishes with regular feeding. This experiment was conducted for Labeo rohita (Hamilton) of different size groups (4-6 cm, 9-11 cm, 14-16 cm and 19-21 cm) at a concentration of clove oil doses 0.01, 0.025, 0.05, 0.1, 0.2 and 0.3 ml/l of water. The above experimental fish were acclimated to laboratory conditions for 2 hours containing 30 litre water-holding aquaria with deep tube well water under laboratory facilities. Required amount of clove oil was taken to fresh water containing plastic bottle and was shacked vigorously. This solution was transferred to the test tank and mixed thoroughly with water to make 0.01 concentration of clove oil. Same procedure were maintained for others five doses of clove oil. Then acclimated fish were transferred to the test container by scoop net. Then serially various size group of fishes were tested. The air supply to the anesthetic bath was removed immediately before introduction of a fish so that clear observations could be made on fish behavior during the induction period. The three stages of induction of anesthesia caused by exposure to clove oil under identical experimental conditions.

Source of Information: J. Agrofor. Environ. 2(1): 1-6, 2008; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It was found that induction time were low (0.48 minutes) at higher doses 0.3 ml/l of clove oil for Labeo rohita and induction time was high (8.35 minutes) at low dose of clove oil 0.01 ml/l. However, the recovery time was low (1.78 minutes) at low doses 0.01 ml/1 of clove oil and recovery time was high (19.50 minutes) at doses 0.3 ml/l for Rohu.

Knowledge Management: Journal