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In Vitro Plant Regeneration from Cultured Cotyledons of Cotton (gossypium Herbaceum L.)

A. N. Chowdhury
Applied Botany Researeh Division, BCSIR Labratories, Rajshahi, Bangladesh.

M. Z. Rahman
Applied Botany Researeh Division, BCSIR Labratories, Rajshahi, Bangladesh.

A. Samad
Applied Botany Researeh Division, BCSIR Labratories, Rajshahi, Bangladesh.

A. K. M. S. Alam
Applied Botany Researeh Division, BCSIR Labratories, Rajshahi, Bangladesh.

S. Khaleda
Applied Botany Researeh Division, BCSIR Labratories, Rajshahi, Bangladesh.

Abstract/ Executive Summary:

The effect of cytokinins on callus proliferation from cotyledons and plantlet development was studied in cotton. The frequency of callus induction was observed on MS medium enriched with a variety of cytokinins in different concentrations. With the increase of cytokinin concentration, the percentage of callus formation, percentage of shoot developing calli and number of shoots/calli were increased. Among the three different cytokinins studied, BA showed the highest performance. The highest percentage of callus (6.55%) and shoot developing calli (5.87%) was obtained on MS with 1.0 mg/l BA. Highest number of shoots (3.02) per calli was observed on MS media supplemented with 1.0 mg/l Kn. The rooting media composed of MS medium, 0.6% agar, sucrose and fortified with 2.0 mg/l NAA induced root development at the highest percentage (41.23%) with maximum number of roots (3.61) per cutting and length of root (3.62 cm) per culture. The plantlets were acclimatized in natural conditions.

Keywords: In vitro, Callus, Cytokinin, Plantlet, Acclimatization

Location:

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Cotton

Objectives:

To find out the effect of cytokinins on callus proliferation and plant regeneration of cotton using cotyledonary explant

Methodology:

Cotyledon explants were excised from 90 days old mature seeds of cotton (Gossypium herbaceum L.) collected from four different places of Bangladesh. The seeds of were thoroughly washed under running tap water and then treated with 1% savlon and 4-5 drops of Tween 80 for about 15 minutes followed by successive three washing with distilled water to make the material free from savlon. Then the seeds were surface sterilized by immersing the seeds in 0.1% HgCl₂ for 7 minutes with gentle shaking. After discarding the HgCl₂, the seeds were washed with sterile distilled water at least for 6- 7 times till every trace of the sterilent was completelremoved. Seeds were blotted dry on sterile filter paper under aseptic condition. After removing seed coat the cotyledons were inoculated aseptically for callus initiation in MS Medium (Murashige and Skoog, 1962) variously supplemented with Benzyl adenine (BA), 6-furfuryl aminopurine (Kn) and 2-isopentyl-adenine (2ip.) in various combinations . All these operation were done inside laminar airflow hood. The pH of the medium was adjusted to 5.8 before autoclaving at 121^oC for 20 minutes. The cultured Petri dishes were incubated in dark cupboard shelf in culture room.

Source of Information: Bangladesh J. Sci. Ind. Res. 46(3), 359-364, 2011

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The result of the present study reveals that in vitro plant regeneration from unorganized callus tissue using cotyledon explants of cotton although take prolong time (6-10 times subculture at 4-6 week intervals) and regeneration frequency is very low but it could be possible to regeneration in in vitro condition. Moreover, these plant lets could be successfully transferred to the natural environments.

Knowledge Management: Journal