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Diallel Analysis of Soluble Protein in Root Nodules and Seeds in Blackgram [vigna Mungo (l.) Hepper]

Rabeya Basari
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

MA Samad
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

AC Deb
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh

MA Khaleque
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

The study was done to see the inheritance of soluble protein in two quantitative characters, such as seeds and nodules per plant in Blackgram [Vigna mungo (L.) Hepper]. Dominance and additivity were detected for soluble protein in both of the characters. Soluble protein in seeds was found to be regulated by partial dominance whereas, the same in nodule character showed over dominance. The asymmetry in the proportion of genes with positive and negative effects in the parents was indicated for soluble protein in both of the characters. The soluble protein in seeds indicated the presence of an excess of dominant genes in the parents. On the other hand, the same in nodules indicated the presence of an equal proportion of dominant and recessive genes. The negative dominant genes were controlling the character whereas, only one group of dominant genes was found to the same condition in both of the characters. This study also suggested that dominance effect over all loci in heterozygous phase was important for the soluble protein in seeds yield per plant but the dominance effect over all loci in heterozygous phase was important for the total amount of soluble protein in nodules. The general combining ability and specific combining ability were found to be significant for the soluble protein. It was noted that the line 20 (E2025) was good general combiner for the same. The crosses 5 (EML013) × 17 (E86325) and 5 (EMLO13) × 20 (E2025) for soluble protein in nodules and 5 (EMLO 13) × 21 (E86309), 17 (E86325) × 21 (E86309) in seeds were found to be the best combiners as judged from the specific combining ability effects.

Keywords: Diallel, Soluble protein, Blackgram

Location: BARI regional station, Ishurdi, Pabna

Start Date: 00-00-2005

End Date:

Program Area: Variety and Species

Commodity: Black gram

Objectives:

To evaluate the nature of gene action, i.e., additivity and dominance inheritance of soluble protein in seed and root nodules of Blackgram.

Methodology:

At first, four lines of Blackgram [Vigna mungo (L.) Hepper] viz. line-5 (EMLO₁₃), line-17 (E86325), line- 20 (E2025) and line-21 (E86309) were collected from BARI regional station, Ishurdi, Pabna, Bangladesh. The experiment was done in the research field of Botanical Garden at University of Rajshahi, Rajshahi, Bangladesh during 2005 following completely randomized block design. The seeds of different line were shown randomly following individual plant randomization in replicated plots. Each plot had twenty five rows and having seven hills per plot. Data were collected on individual plant basis. Root nodules were collected after 60 days from the sowing of seeds. On the other hand, the seeds were collected in mature condition and dried up properly. The collected experimental data were analyzed following two techniques of analyses (A) Biochemical analysis: Protein content of the different lines of root and seed was determined and (B) Biometrical analysis was done following diallel techniques.

Source of Information: Journal of Biodiversity and Environmental Sciences, Vol. 1(2): 7-15, 2011, ISSN 2220-6663

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, the combining ability analysis in Blackgram showed that the variance due to general combining ability (gca) was significant for soluble protein in both the characters was observed same result in chilli. Variance due to Specific combining ability (sca) was also significant for the chemical character in both seeds and nodules. In the present materials gca was greater than sca suggested that inheritance of the character was predominantly governed by additive and additive × additive epistatic components of genetic variance was observed the similar nature of gene action for different yield attributes of Corchorus capsularis, also found the similar results in brinjal and Chilli, respectively. Therefore, in the present investigation for fixation of additive genetic effects for soluble protein in both the characters, recurrent selection may be profitable. It was noted from that the line 20 (E2025) was general combiner for both the characters under study. The best combiner as observed from sca effects for the crosses, were 5 (EMLO 13 ) × 17 (E86325) and 5 (EMLO13) × 20 (E2025) for total amount of soluble protein in nodules per plant and 5 (EMLO 13 ) × 21 (E86309) and 17 (E86325) × 21 (E86309) for total amount of soluble protein in seeds per plant. These crosses may therefore, be utilized for the study of hybrid vigor and fixation of dominant genes in further breeding research.

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