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Induction and Evaluation of Polyploidy in Some Local Potato Varieties of Bangladesh

M Mahbube Alam
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

M Rezaul karim
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

M Abdul Aziz
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

M Monzur Hossain
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Bulbul Ahmed
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Abul Mandal
School of Life Sciences, University of Skovde, SE-541 28, Sweden

Abstract/ Executive Summary:

To improve the yield potential in this present experiment colchicine were applied on sprouting bud and subsequent selected buds of Bangladeshi local potato. Colchicine treatment showed an increase effects in plant height in the variety of Hagrai, Silbilati & Lalpakri and the var. of Challisha showed no increase result besides, negative impact showed in var. Indurkani, JPR, Goforown and Shaita. Number of leaves formation was showed significant positive results in the variety of Hagrai, Shilbilati and Lalpakri showed positive while the var. of Challisha, JPR, Goforown and Shaita were showed negative result at different stage. The variety of Hagrai, Shilbilati and Lalpakri significantly showed more performance for fresh weight of plant and var. Hagrai responded the highest impact at different intervals, besides Challisha, JPR and Goforgown did not responded. The var. of Hagrai and Lalpakri at all the intervals produced highest number of tubers than rest of varieties. Hagrai and Lalpakri were showed the positive yield performance at the 70 DAPs but Silbilati and Lalpakri were showed the positive impact at the 80 DAPs. In all intervals Lalpakri obtained the best performance after Colchicine treatment. The variety Hagrai, Shilbilati and Lalpakri responded significantly in plant height, number of leaves formation and fresh weight potential of plant, though positive but no significant performance in tuber formation and fresh weight production of tubers after colchicine treatment. This might be explained that colchicine treatment enhances plant height, results in more fresh weight of plant and number of leaves, which consequently increase the number of tuber and finally increase total fresh weight of tubers of potato.

Keywords: Colchicines treatment, Polyploidy induction, Potato

Location: Plant Breeding and Gene Engineering Laboratory, University of Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

To find out the effect of colchicine treatment for enhance polyploidy or chromosome doubling of potato as a modern breeding process.

Methodology:

Medium size potato tubers were collected from the local market of different places of countries. The Cultivars of local potato (Bangladesh) e.g. Hagrai, Shilbilati, Indurkani, Challicha, Lalpakri, JPR, Goforgown and Shaita were used as a plant material and Colchicine and 70% ethanol were used as chemical reagent for the present investigation. Hands and equipments were sterilized with 70% ethanol. When the sprout was 3-5 mm. tip 1 mm. was cut by fine razor blade. A piece of blotting paper cut previously into a small triangular shape and inserted into sprout with the help of fine knife and a pair of forceps. Drop of colchicines solution (0.05%) was applied on the projected end of the triangle continuously for two hours. A fresh drop was not applied until the previous one is dried up. The colchicine treated tubers of 8 LPVs were randomly assigned to the inner eight lines in each replication. After plantation, tubers were covered with soil. For presentation of data in the figures, statistical methods were used to quantify the experimental results. Data on different parameters from different treatments were recorded after 40, 50, 60, 70 and 80 day after plantation (DAP). Standard statistical methods were used for calculating the range of variation.

Source of Information: Journal of Biodiversity and Environmental Sciences, Vol. 1( 2): 16-21, 2011, ISSN: 2220-6663

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In this present experiment mostly optimized a comparative picture of various performances between treated and non treated potato plant in the field condition. The study on Plant height, number of leaves formation per plant, fresh weight of plant, tuber formation and fresh weight impact of tubers revealed that with the application of colchicine. In all intervals Lalpakri obtained the best of yield performance and several times the variety of JPR, Challisha, Shaita, Indurkani were showed negative or no response for different parameters after Colchicine treatment. The variety Hagrai, Shilbilati and Lalpakri responded significantly positive in plant height, number of leaves formation and fresh weighty potential of plant but no significant increase result in tuber formation and fresh weight production of tubers. This might be explained that colchicine treatment enhance plant height, which increase number of leaves and fresh weight of plant that was consequently increase the number of tubers per plant, finally resulting more fresh weight of tubers. In this present study was optimized no any morphological appearance, which indicates the change of chromosomal character sticks, that is absent autotetraploid in all varieties of potato. Therefore, alternative methods have to be done for change of chromosomal character sticks and polyploidy induction for varietal improvement of potato.

Knowledge Management: Journal