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Effect of Mutagenic Agents on Seed Germination and Vegetative Growth of Mustard (brassica Rapa)

N. F. Khan
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia.

N. Hassan
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia.

M. R. Islam
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia.

S. K. Biswas
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia.

Abstract/ Executive Summary:

The experiment was conducted to study the effect of mutagens (physical and chemical) on seed germination along with root and shoot lengths of two locally collected varieties of Brassica rapa denoted as variety-1 and variety-2 in in vitro condition. Seeds were treated with various concentrations of mutagenic agents; physical (gamma radiation), chemical (sodium azide) and combined (gamma radiation + sodium azide). The doses of gamma radiation were 100Gy and 200Gy, sodium azide concentrations were 100, 300 and 500ppm and for combination, 100Gy and 200Gy gamma irradiated seeds were further treated with 100, 300 and 500ppm sodium azide solution. After 7 days observation, 100% seed germination obtained from 100Gy gamma irradiated seeds of variety-1 and 500ppm sodium azide treated seeds of variety- 2. 100% seed germination was also found in 7 days in physical and chemical mutagen combinedly treated seeds of variety-1 from 100Gy gamma radiation + 100ppm sodium azide, 100Gy gamma radiation + 300ppm sodium azide and 200Gy gamma radiation + 100ppm sodium azide and in variety-2, from 100Gy gamma radiation + 500ppm sodium azide and 200Gy gamma radiation + 500ppm sodium azide. Maximum increased root length (avg. 3.8cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 300ppm sodium azide) seeds and minimum decreased root length (avg. 1.0 cm) was from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds. Maximum increased shoot length (avg. 4.7cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 100ppm sodium azide) seeds and minimum decreased shoot length (avg. 1.4cm) was obtained from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds.

Keywords: Mutagenic agents; Gamma radiation; Sodium azide; Combined treatment; Germination rate; Root length; Shoot length; Brassica rapa riety-1 and 2.

Location: Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

In this study, the effect of mutagenic agents on Brassica rapa seed germination and root and shoot development due to alteration in genetic material is analyzed.

Methodology:

Two varieties of Brassica rapa seeds were collected from the locally cultivated plants. A portion of these seeds were used to treat by Co60 gamma irradiator from Atomic Energy Research Establishment (AERE), which is a source of gamma radiation. Two different doses 100Gy and 200Gy of gamma radiation were given (Akgun and Tosum, 2004). These seeds were pre-soaked with distilled water and plated on petridish. Another portion of locally collected seeds were treated with sodium azide solution prepared in different concentrations of 100 ppm, 300 ppm and 500 ppm. These seeds were first presoaked in distilled water then immersed into sodium azide solution for three hours. These seeds were washed thoroughly under running tap water (Larik A, 1987). After washing, the seeds were dried in between filter paper folds. These seeds were then ready for plating on germination petridish. To create a modification some portions of locally collected seeds were combined treated. The combined treatment was, gamma radiated seeds of each dose were immersed into sodium azide solution of three different concentrations, after washing seeds were prepared for plating. Petridishes were prepared with sterile blotting papers and distilled water was poured on it to make the blotting paper wet enough for germination. Then seeds of different treatment category (physical, chemical and combined treated) with control group were plated on different petridishes and kept at normal environmental conditions age value of root and shoot length statistical analysis was performed by SPSS software (ver.15.0.1).

Source of Information: Bangladesh J. Sci. Ind. Res. 48(4), 253-256, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Maximum increased root length (avg. 3.8cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 300ppm sodium azide) seeds and minimum decreased root length (avg. 1.0 cm) was from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds. Maximum increased shoot length (avg. 4.7cm) was obtained from variety-1 combined treated (200Gy gamma radiation + 100ppm sodium azide) seeds and minimum decreased shoot length (avg. 1.4cm) was obtained from variety-2 combined treated (100Gy gamma radiation + 100ppm sodium azide) seeds.

Knowledge Management: Journal