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Pathogenic Bacteria, Pesticide Residues and Metal Content in Giant Fresh Water Prawn, Macrobrachium Rosenbergii (de Man) Sold in Local Markets

M. J. NAYEM
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342,Bangladesh.

A. N. M. FAKHRUDDIN
Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka-1342,Bangladesh.

M. A. Z. CHOWDHURY
Institute of Food & Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka- 1344,Bangladesh..

M. K. ALAM
Institute of Food & Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka- 1344, Bangladesh.

Z. FARDOUS
Institute of Food & Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka- 1344,Bangladesh.

H. RASHID
Institute of Food & Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka- 1344,Bangladesh.

M. M. Hossain
Microbiology and Chemical Division, Dhaka Water Supply and Sewerage Authority (DWASA), Asad Gate, Mohammadpur, Dhaka-1207, Bangladesh.

Abstract/ Executive Summary:

The giant fresh water prawn, Macrobrachium rosenbergii (de Man), a leading export item of Bangladesh was studied in terms of pathogenic bacteria, pesticide residues and metal contents. In microbiological analysis, total viable bacteria, total coliform, total faecal coliform, total Salmonella and total Vibrio count were ranged from 6.8 × 10⁴ to 1.6 × 10^6, 1.5 × 10² to 1.8 × 10⁵, 0.0 to 5.0 × 10², 0.0 to 1.8 × 10² and 0.0 to 3.6 × 10² cfu/gm, respectively. Pesticide residue was analyzed by HPLC. Out of six samples studied, one was found to be contaminated with Quinalphos and another with Cypermethrin, where the concentrations were 0.03 and 0.56 ppm, respectively, below the maximum residue limit set by joint FAO/WHO expert committee. Copper (Cu), cadmium (Cd) and lead (Pb) concentrations were measured by atomic absorption spectrophotometer. The range found for Cu and Cd was from 0.01 to 0.11 ppm and 0.01 to 0.06 ppm, respectively, which is below the maximum residue limit set by joint FAO/WHO expert committee (10 ppm for Cu and 0.2 ppm for Cd). Relatively high microbial load and presence of pathogens in all the tested samples, collected from local market revealed that the samples had exposure to an unhealthy environment.

Keywords: Pathogenic bacteria, Macrobrachium rosenbergii, Quinalphos, Cypermethrin, Fresh water prawn

Location:

Start Date:

End Date:

Program Area: Pest Management

Commodity: Prawn

Objectives:

To determine the prevalence of pathogenic bacteria, the residual level of pesticides and metal content in giant fresh water prawn and to compare the status with globally recommended standard

Methodology:

The samples of the giant fresh water prawn used in the experiment were aseptically collected from Savar bazaar, Dhaka and transported to the laboratory as quickly as possible for microbiological analysis. For microbiological analysis five different types of media namely, nutrient agar medium (Difco, USA) for total viable bacterial count, MacConkey agar medium (Difco, USA) for total coli form count, mFC agar medium for total faecal coliform count, Thiosulfate Citrate Bile Salts Sucrose Agar medium (TCBS) for total Vibrio count, andS–S agar medium for total Salmonella-Shigella count were used. Standard plate count (SPC) method was used for inoculation technique, described by APHA (1998). Pesticides from prawn samples were extracted with petroleum ether and then concentrated in a rotary vacuum. Extract was then cleaned with florisil column, eluted with diethyl ether and double distilled n-Hexane. Residual level of pesticides was analyzed with the aid of HPLC having PDA Detector (Column: C18, eluent : acetone: water = 70 : 30, flow rate: 1 ml/min, injection volume: 20 ?l, oven temperature: 30^oC). In case of metal analysis, a total of 11 samples were studied according to Association of Official Agricultural Chemists (AOAC 2005). A graphite furnace atomic absorption spectrometer (GSA- EX- 71 Shimadju, Japan) was used to determine the Cd, Cu and Pb concentrations of samples.

Source of Information: Journal of Bangladesh Academy of Sciences, Vol. 35, No. 1, 91-97, 2011

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Collected prawn samples from local market showed relatively high microbial load and presence of pathogens in the tested samples which indicated that the samples had exposure to an unhealthy environment. One sample was also contaminated with quinalphos and another with cypermethrin, where the concentrations were 0.03 and 0.56 ppm respectively, below the maximum residue limit. The range found for Cu and Cd was from 0.01 to 0.11 ppm and 0.01 to 0.06 ppm, respectively, which was below the maximum limit except sample No.1 for having 0.11 ppm Cu.

Knowledge Management: Journal