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Evaluation of Fungicides on the Growth of Rhizoctonia Solani, Rhizoctonia Oryzae and Rhizoctonia Oryzae- Sativae

M. Ibrahim Khalil
Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh

M. H. Rashid
Bangladesh Krishi Bank, Tangail, Bangladesh

Abstract/ Executive Summary:

The sensitivity of five fungicides, Tilt 250 EC, Bavistin 50 WP, Folicur 250 EW, Contaf 5 EC and Forastin 50 WP were evaluated against the mycelial growth and production of sclerotia of Rhizoctonia solani, Rhizoctonia oryzae and Rhizoctonia oryzae-sativae on PDA plates. The concentrations of each fungicide were 0, 10, 25, 50, 100 ppm. The tested fungicides significantly inhibited the mycelial growth of the pathogens. The fungicide Bavistin and Folicur were the most effective against R. solani and R. oryzae, respectively for inhibiting the mycelial growth. The pathogen R. oryzae-sativae was very sensitive to all the fungicides tested even at 10 ppm concentration.

Keywords: Fungicides, Rhizoctonia spp., Sheath blight complex

Location: Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture

Start Date: 00-02-2007

End Date: 00-05-2007

Program Area: Pest Management

Commodity: Fungicide

Objectives:

To assess the sensitivity of the pathogens to some important fungicides.

Methodology:

Pure culture of Rhizoctonia solani, Rhizoctonia oryzae and Rhizoctonia oryzae- sativae were collected from Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA). The pathogens were purified by hyphal tip method. The pathogens were set to grow in the petridishes containing 2% water agar medium. These petridishes were observed under sterio-microscope and tip of the hyphae along with a small block of medium cut by a thin and sharp sterilized needle. The blocks were immediately transferred in to PDA slants and incubated at 28 ± 2°C for seven days. R. solani, R. oryzae and R. oryzae-sativae were characterized based on the appearance or colour of the colony and the formation pattern, size and shape of the sclerotia or sclerotial crusts. The purified culture thus obtained were kept in a refrigerator (5-6^oC) for subsequent uses. The effectiveness of five fungicides namely Tilt 250 EC (Propiconazole), Bavistin 50 WP (Carbendazim), Folicure 250 EW (Tebuconazole), Contaf 5 EC (Hexaconazole) and Forastin 50 WP (Carbendazim), collected from different agro-chemical companies of Bangladesh were evaluated against R. solani, R. oryzae and R. oryzae-sativae. The experiment was conducted at the laboratory of Plant Pathology Division, BINA, Mymensingh during February to May, 2007. The fungicides were used to evaluate the sensitivity among the Rhizoctonia spp. in terms of responses to chemicals. The concentrations of each fungicide were 0, 10, 25, 50, 100 ppm. Requisite amount of individual fungicide was mixed with PDA before sterilization. Autoclaved PDA was poured in to petridishes (90 mm) at 20 ml per petridish. After solidification of the medium, the plates were inoculated with 5 mm mycelium blocks taken from the edge of three days old colony of each of the R. solani, R. oryzae and R. oryzae-sativae strain grown on PDA. In case of control treatments (0 ppm) water was used instead of chemical. Each treatment was replicated five times. The plates were incubated at 28 ± 2°C. Data on radial mycelial growth of the fungi were recorded at 24 hours interval. Precent growth inhibition (I) = {Radial growth in control (C) - Radial growth in treatments (T) / Radial growth in control (C)} = × 100. Where, I, C and T denote percent growth inhibition, radial growth in control and radial growth in treatment, respectively.

Source of Information: Bangladesh Journal of Nuclear Agriculture. Vol: 29, pp: 105-111, ISSN 0258-7130, 2013

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Based on the findings of the present study, it might be concluded that Bavistin was effective in inhibiting the growth of R. solani. Folicur was effective in inhibiting the growth of R. oryzae. The fungus R. oryzae-sativae was very sensitive to the tested fungicides even at low concentration.

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